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Literature summary for 2.7.6.1 extracted from
- Human phosphoribosylpyrophosphate synthetase. Comparison of purified normal and mutant enzymes (1975), J. Biol. Chem., 250, 6822-6830.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Homo sapiens |  |
| 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | |
| ADP | - |
Homo sapiens | |
| AMP | - |
Homo sapiens | |
| GDP | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ATP | pH conditions not mentioned, 37ºC, wild type and mutant enzyme | Homo sapiens | |
| 0.03 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
| 0.41 | - |
D-ribose 5-phosphate | pH conditions not mentioned, 37ºC, mutant enzyme | Homo sapiens | |
| 0.57 | - |
D-ribose 5-phosphate | pH conditions not mentioned, 37ºC, wild type enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme occurs in multiple states of aggregation | Homo sapiens |
| 32000 | - |
x * 32000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4500fold purification | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | from normal patients and patients with excessive activity of the enzyme | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.65 | - |
wild type enzyme | Homo sapiens |
| 8.17 | - |
mutant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
inactivation of mutant enzyme proceeds faster than that of normal enzyme | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing, mutant enzyme | - |
4.9 |
| Homo sapiens | isoelectric focusing, normal enzyme | - |
5.1 |
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