Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | can partially replace phosphate in activation | Rattus norvegicus | |
phosphate | requirement | Rattus norvegicus | |
sulfate | can partially replace phosphate in activation | Rattus norvegicus |
General Stability | Organism |
---|---|
high enzyme concentration, albumin, 0.05 mg/ml, EDTA, 1 mM or DTT, 1 mM stabilizes during assay | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive inhibition to ATP | Rattus norvegicus | |
AMP | noncompetitive to both substrates | Rattus norvegicus | |
ATP | free form, slight inhibition at 1 mM | Rattus norvegicus | |
D-ribose 5-phosphate | substrate inhibition above 1.5 mM | Rattus norvegicus | |
dADP | competitive to ATP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | pH and temperature conditions not mentioned | Rattus norvegicus | |
0.22 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.29 | - |
5-phospho-D-ribose diphosphate | pH and temperature conditions not mentioned | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required to form a complex with ATP and as a free cation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme is in an aggregated state | Rattus norvegicus |
40000 | - |
x * 40000, SDS-PAGE with mercaptoethanol | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
acetone powder, acid and streptomycin precipitation, heat treatment and agarose chromatography, 1500 to 3000fold purification | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity after 2 months. -20ºC, rapid loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
dATP + D-ribose 5-phosphate | - |
Rattus norvegicus | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE with mercaptoethanol | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
ADP | pH and temperature conditions not mentioned | Rattus norvegicus |