BRENDA - Enzyme Database
show all sequences of 2.7.4.B2

In vitro ATP regeneration from polyphosphate and AMP by polyphosphate:AMP phosphotransferase and adenylate kinase from Acinetobacter johnsonii 210A

Resnick, S.M.; Zehnder, A.J.B.; Appl. Environ. Microbiol. 66, 2045-2051 (2000)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
PPT catalyzes a key reaction in the cell-free regeneration of ATP from AMP and polyphosphate. The PPT/AdK system provides an alternative to existing enzymatic ATP regeneration systems in which phosphoenolpyruvate and acetylphosphate serve as phosphoryl donors and has the advantage that AMP and polyP are stable, inexpensive substrates
Acinetobacter johnsonii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
AMP + [phosphate]n
Acinetobacter johnsonii
-
ADP + [phosphate]n-1
-
-
?
AMP + [phosphate]n
Acinetobacter johnsonii 210A
-
ADP + [phosphate]n-1
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter johnsonii
-
-
-
Acinetobacter johnsonii 210A
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-dAMP + (phosphate)n
-
718620
Acinetobacter johnsonii
2'-dADP + (phosphate)n-1
-
-
-
?
2'-dAMP + (phosphate)n
-
718620
Acinetobacter johnsonii 210A
2'-dADP + (phosphate)n-1
-
-
-
?
2'-deoxy-AMP + [phosphate]n
-
718620
Acinetobacter johnsonii
2'-deoxy-ADP + [phosphate]n-1
-
-
-
?
AMP + (phosphate)n
-
718620
Acinetobacter johnsonii
ADP + (phosphate)n-1
-
-
-
?
AMP + (phosphate)n
-
718620
Acinetobacter johnsonii 210A
ADP + (phosphate)n-1
-
-
-
?
AMP + [phosphate]n
-
718620
Acinetobacter johnsonii
ADP + [phosphate]n-1
-
-
-
?
AMP + [phosphate]n
-
718620
Acinetobacter johnsonii 210A
ADP + [phosphate]n-1
-
-
-
?
additional information
GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates
718620
Acinetobacter johnsonii
?
-
-
-
-
additional information
PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates
718620
Acinetobacter johnsonii
?
-
-
-
-
additional information
GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates
718620
Acinetobacter johnsonii 210A
?
-
-
-
-
additional information
PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates
718620
Acinetobacter johnsonii 210A
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Acinetobacter johnsonii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Acinetobacter johnsonii
Application (protein specific)
Application
Commentary
Organism
synthesis
PPT catalyzes a key reaction in the cell-free regeneration of ATP from AMP and polyphosphate. The PPT/AdK system provides an alternative to existing enzymatic ATP regeneration systems in which phosphoenolpyruvate and acetylphosphate serve as phosphoryl donors and has the advantage that AMP and polyP are stable, inexpensive substrates
Acinetobacter johnsonii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
AMP + [phosphate]n
Acinetobacter johnsonii
-
ADP + [phosphate]n-1
-
-
?
AMP + [phosphate]n
Acinetobacter johnsonii 210A
-
ADP + [phosphate]n-1
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-dAMP + (phosphate)n
-
718620
Acinetobacter johnsonii
2'-dADP + (phosphate)n-1
-
-
-
?
2'-dAMP + (phosphate)n
-
718620
Acinetobacter johnsonii 210A
2'-dADP + (phosphate)n-1
-
-
-
?
2'-deoxy-AMP + [phosphate]n
-
718620
Acinetobacter johnsonii
2'-deoxy-ADP + [phosphate]n-1
-
-
-
?
AMP + (phosphate)n
-
718620
Acinetobacter johnsonii
ADP + (phosphate)n-1
-
-
-
?
AMP + (phosphate)n
-
718620
Acinetobacter johnsonii 210A
ADP + (phosphate)n-1
-
-
-
?
AMP + [phosphate]n
-
718620
Acinetobacter johnsonii
ADP + [phosphate]n-1
-
-
-
?
AMP + [phosphate]n
-
718620
Acinetobacter johnsonii 210A
ADP + [phosphate]n-1
-
-
-
?
additional information
GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates
718620
Acinetobacter johnsonii
?
-
-
-
-
additional information
PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates
718620
Acinetobacter johnsonii
?
-
-
-
-
additional information
GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates
718620
Acinetobacter johnsonii 210A
?
-
-
-
-
additional information
PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates
718620
Acinetobacter johnsonii 210A
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Acinetobacter johnsonii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Acinetobacter johnsonii
General Information
General Information
Commentary
Organism
additional information
ATP regeneration from AMP and polyphophate using PPT, firefly luciferase and hexokinase as model ATP-requiring enzymes, ADP can be converted to ATP by adenylate kinase, AdK. Establishment of a PPT/AdK system for ATP regeneration with coupled hexokinase, overview
Acinetobacter johnsonii
physiological function
PPT from Acinetobacter johnsonii is specific for AMP and 2'-dAMP
Acinetobacter johnsonii
General Information (protein specific)
General Information
Commentary
Organism
additional information
ATP regeneration from AMP and polyphophate using PPT, firefly luciferase and hexokinase as model ATP-requiring enzymes, ADP can be converted to ATP by adenylate kinase, AdK. Establishment of a PPT/AdK system for ATP regeneration with coupled hexokinase, overview
Acinetobacter johnsonii
physiological function
PPT from Acinetobacter johnsonii is specific for AMP and 2'-dAMP
Acinetobacter johnsonii
Other publictions for EC 2.7.4.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737499
Motomura
A new subfamily of polyphospha ...
Meiothermus ruber, Meiothermus ruber NBRC 106122
Appl. Environ. Microbiol.
80
2602-2608
2014
1
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1
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2
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2
2
6
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2
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1
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11
1
1
1
1
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1
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3
3
-
-
-
738411
Jyoti Roy
-
Polyphosphate kinase from Leis ...
Leishmania donovani, Leishmania donovani MHOM/IN/83/AG83
Int. J. Drug Develop. Res.
6
159-164
2014
-
-
-
-
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1
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2
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1
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1
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1
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1
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720887
Zhang
Inorganic polyphosphate in the ...
Myxococcus xanthus
Proc. Natl. Acad. Sci. USA
102
13416-13420
2005
-
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722513
Shiba
Polyphosphate:AMP phosphotrans ...
Acinetobacter johnsonii, Acinetobacter johnsonii 210A
J. Bacteriol.
187
1859-1865
2005
-
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1
-
-
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1
3
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2
1
2
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6
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1
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3
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16
1
1
1
1
3
1
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1
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1
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1
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3
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2
1
2
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1
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3
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16
1
1
1
1
3
1
-
1
-
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1
1
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3
3
720949
Shi
Inorganic polyphosphate in Bac ...
Bacillus cereus
Proc. Natl. Acad. Sci. USA
49
17061-17065
2004
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722511
Itoh
Polyphosphate synthetic activi ...
Acinetobacter johnsonii, Acinetobacter johnsonii 210A
J. Bacteriol.
186
5178-5181
2004
-
-
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-
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2
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1
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7
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5
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14
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1
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1
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2
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1
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7
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14
-
1
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-
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1
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-
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645130
Kameda
A novel ATP regeneration syste ...
Acinetobacter johnsonii, Myxococcus xanthus, Myxococcus xanthus DK101
J. Biosci. Bioeng.
91
557-563
2001
-
2
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2
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1
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1
1
8
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2
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2
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718728
Ishige
Polyphosphate:AMP phosphotrans ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
281
821-826
2001
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1
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1
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718766
Tanaka
-
A sensitive method for detecti ...
Acinetobacter johnsonii
Biochem. Eng. J.
9
193-197
2001
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4
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718620
Resnick
In vitro ATP regeneration from ...
Acinetobacter johnsonii, Acinetobacter johnsonii 210A
Appl. Environ. Microbiol.
66
2045-2051
2000
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11
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1
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1
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2
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718947
Shiba
-
Inorganic polyphosphate and po ...
Escherichia coli
Biochemistry
65
375-384
2000
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720883
Ishige
Inorganic polyphosphate kinase ...
Escherichia coli, Escherichia coli JM109
Proc. Natl. Acad. Sci. USA
97
14168-14171
2000
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4
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721769
Bonting
Purification and properties of ...
Acinetobacter johnsonii, Acinetobacter johnsonii 210A
Biodegradation
10
393-398
1999
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722288
Bark
Polyphosphate-dependent enzyme ...
Cellulosimicrobium cellulans, no activity in Aureobacterium saperdae, no activity in Curtobacterium sp., no activity in Shewanella putrefaciens
FEMS Microbiol. Lett.
107
133-138
1993
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719681
Bonting
Properties of polyphosphate:AM ...
Acinetobacter johnsonii, Acinetobacter johnsonii 210A, Acinetobacter sp., Acinetobacter sp. 210A
J. Bacteriol.
173
6484-6488
1991
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4
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718617
van Groenstijn
Polyphosphate-degrading enzyme ...
Acinetobacter sp.
Appl. Environ. Microbiol.
55
219-223
1989
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