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Literature summary for 2.7.4.9 extracted from
- Molecular characterization, heterologous expression and kinetic analysis of recombinant Plasmodium falciparum thymidylate kinase (2008), J. Biochem., 144, 245-250.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dAMP | 5 mM, about 15% inhibition | Plasmodium falciparum |  |
| dUMP | 5 mM, about 15% inhibition | Plasmodium falciparum | |
| EDTA | 4 mM, 80% loss of activity | Plasmodium falciparum | |
| additional information | not inhibitory: 2-mercaptoethanol up to 5 mM, AMP, CMP, dCMP, GMP and dGMP and | Plasmodium falciparum | |
| UMP | 5 mM, about 15% inhibition | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
dTMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 0.03 | - |
dGMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 0.577 | - |
dIMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 0.78 | - |
dUMP | pH 7.2, 30°C | Plasmodium falciparum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
substrate dIMP, pH 7.2, 30°C | Plasmodium falciparum |
| 6.5 | - |
substrate dUMP, pH 7.2, 30°C | Plasmodium falciparum |
| 13 | - |
substrate dGMP, pH 7.2, 30°C | Plasmodium falciparum |
| 15 | - |
substrate dTMP, pH 7.2, 30°C | Plasmodium falciparum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 months, no loss of activity | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dGMP | 86% of the activity with dTMP | Plasmodium falciparum | ADP + dGDP | - |
? | |
| ATP + dIMP | 15% of the activity with dTMP | Plasmodium falciparum | ADP + dIDP | - |
? | |
| ATP + dTMP | - |
Plasmodium falciparum | ADP + dTDP | - |
? | |
| ATP + dUMP | 43% of the activity with dTMP | Plasmodium falciparum | ADP + dUDP | - |
? | |
| ATP + GMP | 1.5% of the activity with dTMP | Plasmodium falciparum | ADP + GDP | - |
? | |
| dATP + dTMP | dATP, 64% of the activity with ATP | Plasmodium falciparum | dADP + dTDP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 30% residual activity | Plasmodium falciparum |
| 65 | - |
10 min, complete loss of activity | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
dIMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 2.9 | - |
dGMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.4 | - |
dTMP | pH 7.2, 30°C | Plasmodium falciparum | |
| 4.1 | - |
dUMP | pH 7.2, 30°C | Plasmodium falciparum | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
marked drop in activity below | Plasmodium falciparum |
| 7 | 9 | - |
Plasmodium falciparum |
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