BRENDA - Enzyme Database show
show all sequences of 2.7.4.8

Diversity in guanosine 3,5-bisdiphosphate (ppGpp) sensitivity among guanylate kinases of bacteria and plants

Nomura, Y.; Izumi, A.; Fukunaga, Y.; Kusumi, K.; Iba, K.; Watanabe, S.; Nakahira, Y.; Weber, A.P.; Nozawa, A.; Tozawa, Y.; J. Biol. Chem. 289, 15631-15641 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bacillus subtilis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Escherichia coli
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Synechococcus elongatus
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3); recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Oryza sativa Japonica Group
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3); recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pisum sativum
Inhibitors
Inhibitors
Commentary
Organism
Structure
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Arabidopsis thaliana
guanosine 3',5'-bisdiphosphate
specific inhibition of the isozyme
Bacillus subtilis
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Oryza sativa Japonica Group
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Pisum sativum
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Escherichia coli
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Oryza sativa Japonica Group
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Pisum sativum
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Saccharomyces cerevisiae
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Synechococcus elongatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.073
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
0.156
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
0.165
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
0.202
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
chloroplast
-
Oryza sativa Japonica Group
9507
-
chloroplast
-
Pisum sativum
9507
-
cytosol
-
Escherichia coli
5829
-
cytosol
-
Oryza sativa Japonica Group
5829
-
cytosol
-
Pisum sativum
5829
-
cytosol
-
Saccharomyces cerevisiae
5829
-
cytosol
-
Synechococcus elongatus
5829
-
additional information
-
Bacillus subtilis
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Arabidopsis thaliana
Mg2+
required
Bacillus subtilis
Mg2+
required
Escherichia coli
Mg2+
required; required
Oryza sativa Japonica Group
Mg2+
required; required
Pisum sativum
Mg2+
required
Synechococcus elongatus
Mg2+
required
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + GMP
Bacillus subtilis
-
ADP + GDP
-
-
?
ATP + GMP
Escherichia coli
-
ADP + GDP
-
-
?
ATP + GMP
Synechococcus elongatus
-
ADP + GDP
-
-
?
ATP + GMP
Saccharomyces cerevisiae
-
ADP + GDP
-
-
?
ATP + GMP
Pisum sativum
-
ADP + GDP
-
-
?
ATP + GMP
Oryza sativa Japonica Group
-
ADP + GDP
-
-
?
ATP + GMP
Arabidopsis thaliana
-
ADP + GDP
-
-
?
ATP + GMP
Oryza sativa Japonica Group Nipponbare
-
ADP + GDP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q94JM2
-
-
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Oryza sativa Japonica Group
Q10M74
-
-
Oryza sativa Japonica Group
Q2QPW1
-
-
Oryza sativa Japonica Group Nipponbare
Q10M74
-
-
Oryza sativa Japonica Group Nipponbare
Q2QPW1
-
-
Pisum sativum
W8VNI6
cv. Toyonari, gene PsGKpm
-
Pisum sativum
W8VZ39
cv. Toyonari, gene PsGKc
-
Saccharomyces cerevisiae
P15454
-
-
Synechococcus elongatus
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Arabidopsis thaliana
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Bacillus subtilis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Saccharomyces cerevisiae
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Synechococcus elongatus
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography; recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Oryza sativa Japonica Group
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography; recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography, native enzyme from chloroplasts by gel filtration
Pisum sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
leaf
;
Oryza sativa Japonica Group
-
leaf
;
Pisum sativum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + GMP
-
738614
Bacillus subtilis
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Escherichia coli
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Synechococcus elongatus
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Saccharomyces cerevisiae
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Pisum sativum
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Oryza sativa Japonica Group
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Arabidopsis thaliana
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Oryza sativa Japonica Group Nipponbare
ADP + GDP
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
30
-
assay at
Bacillus subtilis
30
-
assay at
Escherichia coli
30
-
assay at; assay at
Oryza sativa Japonica Group
30
-
assay at; assay at
Pisum sativum
30
-
assay at
Saccharomyces cerevisiae
30
-
assay at
Synechococcus elongatus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
23
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
52
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
70
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
209
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Arabidopsis thaliana
7.5
-
assay at
Bacillus subtilis
7.5
-
assay at
Escherichia coli
7.5
-
assay at; assay at
Oryza sativa Japonica Group
7.5
-
assay at; assay at
Pisum sativum
7.5
-
assay at
Saccharomyces cerevisiae
7.5
-
assay at
Synechococcus elongatus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Arabidopsis thaliana
ATP
-
Bacillus subtilis
ATP
-
Escherichia coli
ATP
;
Oryza sativa Japonica Group
ATP
;
Pisum sativum
ATP
-
Synechococcus elongatus
ATP
-
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0028
-
guanosine 3',5'-bisdiphosphate
versus GMP, pH 7.5, 30C
Oryza sativa Japonica Group
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.01
-
versus GMP, pH 7.5, 30C
Oryza sativa Japonica Group
guanosine 3',5'-bisdiphosphate
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bacillus subtilis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Escherichia coli
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Oryza sativa Japonica Group
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pisum sativum
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Synechococcus elongatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Arabidopsis thaliana
ATP
-
Bacillus subtilis
ATP
-
Escherichia coli
ATP
-
Oryza sativa Japonica Group
ATP
-
Pisum sativum
ATP
-
Synechococcus elongatus
ATP
-
Saccharomyces cerevisiae
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.01
-
versus GMP, pH 7.5, 30C
Oryza sativa Japonica Group
guanosine 3',5'-bisdiphosphate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Arabidopsis thaliana
guanosine 3',5'-bisdiphosphate
specific inhibition of the isozyme
Bacillus subtilis
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Oryza sativa Japonica Group
guanosine 3',5'-bisdiphosphate
specific inhibition of the organellar isozyme
Pisum sativum
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Escherichia coli
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Oryza sativa Japonica Group
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Pisum sativum
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Saccharomyces cerevisiae
additional information
no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate
Synechococcus elongatus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0028
-
guanosine 3',5'-bisdiphosphate
versus GMP, pH 7.5, 30C
Oryza sativa Japonica Group
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.073
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
0.156
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
0.165
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
0.202
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
chloroplast
-
Oryza sativa Japonica Group
9507
-
chloroplast
-
Pisum sativum
9507
-
cytosol
-
Escherichia coli
5829
-
cytosol
-
Oryza sativa Japonica Group
5829
-
cytosol
-
Pisum sativum
5829
-
cytosol
-
Saccharomyces cerevisiae
5829
-
cytosol
-
Synechococcus elongatus
5829
-
additional information
-
Bacillus subtilis
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Arabidopsis thaliana
Mg2+
required
Bacillus subtilis
Mg2+
required
Escherichia coli
Mg2+
required
Oryza sativa Japonica Group
Mg2+
required
Pisum sativum
Mg2+
required
Synechococcus elongatus
Mg2+
required
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + GMP
Bacillus subtilis
-
ADP + GDP
-
-
?
ATP + GMP
Escherichia coli
-
ADP + GDP
-
-
?
ATP + GMP
Synechococcus elongatus
-
ADP + GDP
-
-
?
ATP + GMP
Saccharomyces cerevisiae
-
ADP + GDP
-
-
?
ATP + GMP
Pisum sativum
-
ADP + GDP
-
-
?
ATP + GMP
Oryza sativa Japonica Group
-
ADP + GDP
-
-
?
ATP + GMP
Arabidopsis thaliana
-
ADP + GDP
-
-
?
ATP + GMP
Oryza sativa Japonica Group Nipponbare
-
ADP + GDP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Arabidopsis thaliana
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Bacillus subtilis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Oryza sativa Japonica Group
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Pisum sativum
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Saccharomyces cerevisiae
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
Synechococcus elongatus
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography, native enzyme from chloroplasts by gel filtration
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
leaf
-
Oryza sativa Japonica Group
-
leaf
-
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + GMP
-
738614
Bacillus subtilis
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Escherichia coli
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Synechococcus elongatus
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Saccharomyces cerevisiae
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Pisum sativum
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Oryza sativa Japonica Group
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Arabidopsis thaliana
ADP + GDP
-
-
-
?
ATP + GMP
-
738614
Oryza sativa Japonica Group Nipponbare
ADP + GDP
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
30
-
assay at
Bacillus subtilis
30
-
assay at
Escherichia coli
30
-
assay at
Oryza sativa Japonica Group
30
-
assay at
Pisum sativum
30
-
assay at
Saccharomyces cerevisiae
30
-
assay at
Synechococcus elongatus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
23
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
52
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
70
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
209
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Arabidopsis thaliana
7.5
-
assay at
Bacillus subtilis
7.5
-
assay at
Escherichia coli
7.5
-
assay at
Oryza sativa Japonica Group
7.5
-
assay at
Pisum sativum
7.5
-
assay at
Saccharomyces cerevisiae
7.5
-
assay at
Synechococcus elongatus
General Information
General Information
Commentary
Organism
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Arabidopsis thaliana
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Bacillus subtilis
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview. Accumulation of guanosine 3',5'-bisdiphosphate has little effect on the guanine nucleotide profile of Escherichia coli
Escherichia coli
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview; guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Oryza sativa Japonica Group
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview; guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Pisum sativum
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Saccharomyces cerevisiae
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Synechococcus elongatus
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Arabidopsis thaliana
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol; guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Oryza sativa Japonica Group
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development; guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition of GKpm activity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Pisum sativum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Arabidopsis thaliana
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Bacillus subtilis
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview. Accumulation of guanosine 3',5'-bisdiphosphate has little effect on the guanine nucleotide profile of Escherichia coli
Escherichia coli
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Oryza sativa Japonica Group
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Pisum sativum
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Saccharomyces cerevisiae
metabolism
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview
Synechococcus elongatus
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Arabidopsis thaliana
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Oryza sativa Japonica Group
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development
Pisum sativum
physiological function
guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition of GKpm activity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol
Pisum sativum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
314
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
317
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
344
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
1347
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
314
-
GMP
recombinant isozyme, pH 7.5, 30C
Bacillus subtilis
317
-
GMP
recombinant chloroplast isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
344
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Oryza sativa Japonica Group
1347
-
GMP
recombinant cytosolic isozyme, pH 7.5, 30C
Escherichia coli
Other publictions for EC 2.7.4.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738145
Jain
Insights into open/closed conf ...
Homo sapiens
Eur. Biophys. J.
45
81-89
2016
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739667
Zhang
Mechanistic insight into the f ...
Saccharomyces cerevisiae
Sci. Rep.
5
8405
2015
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738614
Nomura
Diversity in guanosine 3,5-bis ...
Arabidopsis thaliana, Bacillus subtilis, Escherichia coli, Oryza sativa Japonica Group, Oryza sativa Japonica Group Nipponbare, Pisum sativum, Saccharomyces cerevisiae, Synechococcus elongatus
J. Biol. Chem.
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15631-15641
2014
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739240
Gupta
Purification and characterizat ...
Brugia malayi
Parasitology
141
1341-1352
2014
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2
737590
Mori
Crystal structure of the guany ...
Homo sapiens
Biochem. Biophys. Res. Commun.
435
334-338
2013
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Dembowski
Alternative splicing of a nove ...
Homo sapiens
J. Nucleic Acids
2012
816237
2012
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721551
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Point mutations in human guany ...
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Biochem. Pharmacol.
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131-138
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722117
Zhu
Guanylate kinase domains of th ...
Homo sapiens, Rattus norvegicus
EMBO J.
30
4986-4997
2011
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2
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723014
Kandeel
Binding dynamics and energetic ...
Plasmodium falciparum
J. Mol. Recognit.
24
322-332
2011
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700079
Kandeel
Molecular cloning, expression, ...
Plasmodium falciparum
Mol. Biochem. Parasitol.
159
130-133
2008
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11
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700475
Gardoni
Postsynaptic density-membrane ...
Homo sapiens, Mus musculus, Rattus norvegicus
Neuroscience
158
324-333
2008
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700950
Gonzalez-Gutierrez
The guanylate kinase domain of ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
105
14198-14203
2008
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672643
Choi
Guanylate kinase, induced fit, ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biophys. J.
92
1651-1658
2007
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675510
Sumita
Synaptic scaffolding molecule ...
Rattus norvegicus
J. Neurochem.
100
154-166
2007
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676138
Reese
The guanylate kinase domain of ...
Rattus norvegicus
Nat. Struct. Mol. Biol.
14
155-163
2007
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700744
Sugimoto
The rice nuclear gene, VIRESCE ...
Oryza sativa
Plant J.
52
512-527
2007
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671155
El Omari
Structure of Staphylococcus au ...
Staphylococcus aureus
Acta Crystallogr. Sect. F
F62
949-953
2006
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672440
Hible
Crystal structures of GMP kina ...
Escherichia coli
Biochimie
88
1157-1164
2006
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673847
Willmon
A guanylate kinase/HSV-1 thymi ...
Homo sapiens, Mus musculus
Gene Ther.
13
1309-1312
2006
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675894
Abergel
Impact of the excision of an a ...
Rickettsia conorii
Mol. Biol. Evol.
23
2112-2122
2006
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662657
Hible
Calorimetric and crystallograp ...
Escherichia coli
J. Mol. Biol.
352
1044-1059
2005
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660690
Stolworthy
A novel Escherichia coli strai ...
Escherichia coli, Escherichia coli TS202A
Anal. Biochem.
322
40-47
2003
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662176
Olsen
Functional analysis of the nuc ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
278
6873-6878
2003
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642707
Sekulic
Structural characterization of ...
Mus musculus
J. Biol. Chem.
277
30236-30243
2002
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642705
Blaszczyk
Crystal structure of unligated ...
Saccharomyces cerevisiae
J. Mol. Biol.
307
247-257
2001
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1
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642706
Stolworthy
The mouse guanylate kinase dou ...
Mus musculus
Protein Eng.
14
903-909
2001
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1
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3
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Prinz
Binding of nucleotides to guan ...
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Refined structure of the compl ...
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Guanylate kinase from Saccharo ...
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Phosphorylation of the antivir ...
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Moriguchi
Purification and properties of ...
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Oeschger
Guanylate kinase from Escheric ...
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Agarwal
Guanylate kinases from human e ...
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Agarwal
Inhibition of rat hepatic guan ...
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Shimono
Metabolism of deoxyribonucleot ...
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22
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27
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Buccino
Partial purification and prope ...
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Hiraga
Nucleoside monophosphokinases ...
Escherichia coli, Escherichia coli JE24F+
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114
416-418
1966
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Griffith
The partial purification of de ...
Mus musculus
Biochim. Biophys. Acta
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1
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