Any feedback?
Literature summary for 2.7.4.8 extracted from
- Functional analysis of the nucleotide binding domain of membrane-associated guanylate kinases (2003), J. Biol. Chem., 278, 6873-6878.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharomyces cerevisiae |
| PSD-95, containing SH3GK residues 417-724 and CASK, containing SH3GK residues 591-909, expression in Escherichia coli | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P564S/S631D | PSD-95 construct | Rattus norvegicus |
| S35P | reduced activity | Saccharomyces cerevisiae |
| S35P/D101S | reduced activity | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
GMP | mutant S35P | Saccharomyces cerevisiae |  |
| 0.0025 | - |
GMP | mutant S35P/D101S | Saccharomyces cerevisiae | |
| 0.041 | - |
GMP | wildtype | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GMP | Rattus norvegicus | regulation of cellular adhesion and signal transduction at sites of cell-cell contact | ADP + GDP | - |
? | |
| ATP + GMP | Saccharomyces cerevisiae | regulation of cellular adhesion and signal transduction at sites of cell-cell contact | ADP + GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Rattus norvegicus |
| recombinant protein | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
mutant S35P | Saccharomyces cerevisiae |
| 2.5 | - |
mutant S35P/D101S | Saccharomyces cerevisiae |
| 1581 | - |
wildtype | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GMP | - |
Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | regulation of cellular adhesion and signal transduction at sites of cell-cell contact | Rattus norvegicus | ADP + GDP | - |
? | |
| ATP + GMP | regulation of cellular adhesion and signal transduction at sites of cell-cell contact | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | CASK construct has no activity | Rattus norvegicus | ADP + GDP | - |
? | |
| ATP + GMP | PSD-95 construct has no activity | Rattus norvegicus | ADP + GDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GK | - |
Rattus norvegicus |
| MAGUK | - |
Rattus norvegicus |
| MAGUK | - |
Saccharomyces cerevisiae |
| membrane-associated guanylate kinase | - |
Rattus norvegicus |
| membrane-associated guanylate kinase | - |
Saccharomyces cerevisiae |
| YGK | - |
Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
