BRENDA - Enzyme Database show
show all sequences of 2.7.4.8

Metabolism of deoxyribonucleotides. Purification and properties of deoxyguanosine monophosphokinase of calf thymus

Shimono, H.; Sugino, Y.; Eur. J. Biochem. 19, 256-263 (1971)

Data extracted from this reference:

General Stability
General Stability
Organism
dialysis against water, pH 6 with several changes of outer fluid inactivates, stable to dialysis for less than 36 h without changing the outer fluid
Bos taurus
dilution inactivates calf thymus enzyme, KCl protects, not bovine serum albumin
Bos taurus
KCl does not prevent heat inactivation
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
dGMP
competitive inhibitor to GMP
Bos taurus
GMP
competitive inhibitor to dGMP
Bos taurus
Li+
-
Bos taurus
Li+
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
GMP
-
Bos taurus
0.4
-
dGMP
-
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation
Bos taurus
Ca2+
activation
Rattus norvegicus
Co2+
-
Bos taurus
Co2+
-
Rattus norvegicus
Fe2+
no activator
Bos taurus
Fe3+
no activator
Bos taurus
K+
activation; dGMP, not GMP as substrate
Bos taurus
K+
activation; dGMP, not GMP as substrate
Rattus norvegicus
Mg2+
requirement
Bos taurus
Mg2+
requirement
Rattus norvegicus
Mn2+
requirement
Bos taurus
Mn2+
requirement
Rattus norvegicus
additional information
GMP-kinase activity is less sensitive to metal ions than dGMP-kinase activity
Bos taurus
additional information
-
Rattus norvegicus
Na+
activation
Bos taurus
NH4+
activation; less effective than K+
Bos taurus
NH4+
activation; less effective than K+
Rattus norvegicus
Ni2+
activation
Bos taurus
Rb+
-
Bos taurus
Rb+
-
Rattus norvegicus
Zn2+
-
Bos taurus
Zn2+
-
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
calf
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bone marrow
-
Rattus norvegicus
-
brain
-
Bos taurus
-
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Bos taurus
-
liver
-
Rattus norvegicus
-
thymus
-
Bos taurus
-
thymus
-
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
4C, calf thymus enzyme, stable in the absence of thiols
Bos taurus
4C, diluted calf thymus enzyme solution, 0.002 mg/ml, inactivation within 10 days, KCl protects
Bos taurus
4C, partially purified calf thymus enzyme preparation in the presence of 1 M KCl, 3 months
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dGMP
-
642685
Rattus norvegicus
ADP + dGDP
-
-
-
r
ATP + dGMP
-
642685
Bos taurus
ADP + dGDP
-
-
-
r
ATP + GMP
specificity
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
specificity
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
specificity
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
no donor substrates are ITP, dGTP, dCTP or dTTP
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
no donor substrates are ITP, dGTP, dCTP or dTTP
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dCMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
dCMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dCMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dTMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dTMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
AMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
AMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
AMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dAMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
dAMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dAMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
no donor substrates are GTP, CTP, UTP
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
no donor substrates are GTP, CTP, UTP
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Bos taurus
ADP + GDP
-
-
-
r
dATP + dGMP
-
642685
Rattus norvegicus
dADP + dGDP
-
-
-
?
dATP + dGMP
as good as ATP
642685
Bos taurus
dADP + dGDP
-
-
-
?
dATP + GMP
-
642685
Rattus norvegicus
dADP + GDP
-
-
-
?
dATP + GMP
as good as ATP
642685
Bos taurus
dADP + GDP
-
-
-
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation)
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
10
dGMP-phosphorylation
Bos taurus
7
10.9
GMP-phosphorylation
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10.9
about half-maximal activity at pH 5 and maximal activity at pH 10.9, GMP-phosphorylation, calf thymus
Bos taurus
5.7
10.9
about half-maximal activity at pH 5.7 and 10.9, dGMP-phosphorylation, calf thymus
Bos taurus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.064
-
GMP
pH 7.5
Bos taurus
0.57
-
dGMP
pH 7.5
Bos taurus
General Stability (protein specific)
General Stability
Organism
dialysis against water, pH 6 with several changes of outer fluid inactivates, stable to dialysis for less than 36 h without changing the outer fluid
Bos taurus
dilution inactivates calf thymus enzyme, KCl protects, not bovine serum albumin
Bos taurus
KCl does not prevent heat inactivation
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dGMP
competitive inhibitor to GMP
Bos taurus
GMP
competitive inhibitor to dGMP
Bos taurus
Li+
-
Bos taurus
Li+
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.064
-
GMP
pH 7.5
Bos taurus
0.57
-
dGMP
pH 7.5
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
GMP
-
Bos taurus
0.4
-
dGMP
-
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation
Bos taurus
Ca2+
activation
Rattus norvegicus
Co2+
-
Bos taurus
Co2+
-
Rattus norvegicus
Fe2+
no activator
Bos taurus
Fe3+
no activator
Bos taurus
K+
activation; dGMP, not GMP as substrate
Bos taurus
K+
activation; dGMP, not GMP as substrate
Rattus norvegicus
Mg2+
requirement
Bos taurus
Mg2+
requirement
Rattus norvegicus
Mn2+
requirement
Bos taurus
Mn2+
requirement
Rattus norvegicus
additional information
GMP-kinase activity is less sensitive to metal ions than dGMP-kinase activity
Bos taurus
additional information
-
Rattus norvegicus
Na+
activation
Bos taurus
NH4+
activation; less effective than K+
Bos taurus
NH4+
activation; less effective than K+
Rattus norvegicus
Ni2+
activation
Bos taurus
Rb+
-
Bos taurus
Rb+
-
Rattus norvegicus
Zn2+
-
Bos taurus
Zn2+
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bone marrow
-
Rattus norvegicus
-
brain
-
Bos taurus
-
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Bos taurus
-
liver
-
Rattus norvegicus
-
thymus
-
Bos taurus
-
thymus
-
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, calf thymus enzyme, stable in the absence of thiols
Bos taurus
4C, diluted calf thymus enzyme solution, 0.002 mg/ml, inactivation within 10 days, KCl protects
Bos taurus
4C, partially purified calf thymus enzyme preparation in the presence of 1 M KCl, 3 months
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dGMP
-
642685
Rattus norvegicus
ADP + dGDP
-
-
-
r
ATP + dGMP
-
642685
Bos taurus
ADP + dGDP
-
-
-
r
ATP + GMP
specificity
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
specificity
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
specificity
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
no donor substrates are ITP, dGTP, dCTP or dTTP
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
no donor substrates are ITP, dGTP, dCTP or dTTP
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dCMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
dCMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dCMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dTMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dTMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
AMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
AMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
AMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
dAMP is no acceptor substrate
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
dAMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
dAMP is no acceptor substrate
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
no donor substrates are GTP, CTP, UTP
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
no donor substrates are GTP, CTP, UTP
642685
Bos taurus
ADP + GDP
-
-
-
r
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Rattus norvegicus
ADP + GDP
-
-
-
?
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Bos taurus
ADP + GDP
-
-
-
?
ATP + GMP
CMP, UMP are no acceptor substrates
642685
Bos taurus
ADP + GDP
-
-
-
r
dATP + dGMP
-
642685
Rattus norvegicus
dADP + dGDP
-
-
-
?
dATP + dGMP
as good as ATP
642685
Bos taurus
dADP + dGDP
-
-
-
?
dATP + GMP
-
642685
Rattus norvegicus
dADP + GDP
-
-
-
?
dATP + GMP
as good as ATP
642685
Bos taurus
dADP + GDP
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation)
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
10
dGMP-phosphorylation
Bos taurus
7
10.9
GMP-phosphorylation
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10.9
about half-maximal activity at pH 5 and maximal activity at pH 10.9, GMP-phosphorylation, calf thymus
Bos taurus
5.7
10.9
about half-maximal activity at pH 5.7 and 10.9, dGMP-phosphorylation, calf thymus
Bos taurus
Other publictions for EC 2.7.4.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738145
Jain
Insights into open/closed conf ...
Homo sapiens
Eur. Biophys. J.
45
81-89
2016
-
-
1
-
-
-
1
-
-
1
1
1
-
2
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
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1
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
739667
Zhang
Mechanistic insight into the f ...
Saccharomyces cerevisiae
Sci. Rep.
5
8405
2015
-
-
-
-
1
-
-
-
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1
-
1
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1
-
-
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-
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-
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2
-
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-
-
-
-
-
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1
-
-
-
-
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1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
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-
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1
1
-
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-
738614
Nomura
Diversity in guanosine 3,5-bis ...
Arabidopsis thaliana, Bacillus subtilis, Escherichia coli, Oryza sativa Japonica Group, Oryza sativa Japonica Group Nipponbare, Pisum sativum, Saccharomyces cerevisiae, Synechococcus elongatus
J. Biol. Chem.
289
15631-15641
2014
-
-
7
-
-
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9
4
9
7
-
11
-
20
-
-
7
-
-
3
-
-
11
-
7
-
-
4
7
-
-
7
1
-
1
-
-
9
9
-
-
-
1
9
1
4
9
9
-
11
-
-
-
9
-
5
-
-
11
-
9
-
-
4
9
-
-
-
-
10
14
-
4
4
739240
Gupta
Purification and characterizat ...
Brugia malayi
Parasitology
141
1341-1352
2014
-
-
1
-
-
-
8
2
-
2
2
1
-
3
-
-
1
1
-
-
-
-
6
2
1
1
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
8
-
2
-
2
2
1
-
-
-
1
-
-
-
-
6
2
1
1
-
2
1
1
-
-
-
1
1
-
2
2
737590
Mori
Crystal structure of the guany ...
Homo sapiens
Biochem. Biophys. Res. Commun.
435
334-338
2013
-
-
1
1
-
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-
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2
-
-
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3
-
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1
-
-
-
-
-
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1
-
1
-
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-
-
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2
-
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-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
723031
Dembowski
Alternative splicing of a nove ...
Homo sapiens
J. Nucleic Acids
2012
816237
2012
-
-
-
-
-
-
-
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1
-
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3
-
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-
-
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-
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-
-
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-
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-
-
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-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721551
Mertlikova-Kaiserova
Point mutations in human guany ...
Homo sapiens
Biochem. Pharmacol.
82
131-138
2011
-
-
1
-
1
-
-
2
-
-
1
-
-
2
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
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-
-
1
-
-
1
-
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-
-
2
-
-
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
722117
Zhu
Guanylate kinase domains of th ...
Homo sapiens, Rattus norvegicus
EMBO J.
30
4986-4997
2011
-
-
2
2
-
-
-
-
2
-
-
-
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2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
2
-
2
-
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-
-
2
-
-
-
-
-
-
2
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Purification and properties of ...
Bos taurus
Eur. J. Biochem.
161
551-556
1986
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642698
Boehme
Phosphorylation of the antivir ...
Homo sapiens
J. Biol. Chem.
259
12346-12349
1984
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642690
Moriguchi
Purification and properties of ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
662
165-167
1981
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642687
Oeschger
Guanylate kinase from Escheric ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
51
473-482
1978
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6
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1
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178
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642688
Agarwal
Guanylate kinases from human e ...
Homo sapiens, Rattus norvegicus, Sus scrofa
Methods Enzymol.
51
483-490
1978
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12
14
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1
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37
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642695
Agarwal
Inhibition of rat hepatic guan ...
Rattus norvegicus
Biochem. Pharmacol.
24
791-795
1975
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642685
Shimono
Metabolism of deoxyribonucleot ...
Bos taurus, Rattus norvegicus
Eur. J. Biochem.
19
256-263
1971
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3
4
2
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27
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27
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642686
Buccino
Partial purification and prope ...
Rattus norvegicus
Arch. Biochem. Biophys.
132
49-61
1969
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10
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15
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642699
Hiraga
Nucleoside monophosphokinases ...
Escherichia coli, Escherichia coli JE24F+
Biochim. Biophys. Acta
114
416-418
1966
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642684
Griffith
The partial purification of de ...
Mus musculus
Biochim. Biophys. Acta
108
114-124
1965
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