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Literature summary for 2.7.4.7 extracted from

  • Cheng, G.; Bennett, E.M.; Begley, T.P.; Ealick, S.E.
    Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3.ANG. resolution (2002), Structure, 10, 225-235.
    View publication on PubMed
Search for more literature for 2.7.4.7

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion, 6 mg/ml HMPP kinase, hanging drops contain 0.002 mL protein solution and 0.002 ml reservoir solution, crystal without HMP are obtained using a reservoir solution containing 1.35 M MgSO4, 150 mM MES, pH 7.0, crystals with HMP are obtained with 1.4 M MgSO4 and 150 M MES, crystals of native HMP-P kinase diffract to 2.3 A resolution Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HMP-P kinase Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine proposed reaction mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
 ?

Subunits

Subunits Comment Organism
dimer crystallization data Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
HMP-P kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
ThiD
-
 Salmonella enterica subsp. enterica serovar Typhimurium