Literature summary for 2.7.4.6 extracted from

Yonezawa, Y.; Nagayama, A.; Tokunaga, H.; Ishibashi, M.; Arai, S.; Kuroki, R.; Watanabe, K.; Arakawa, T.; Tokunaga, M.
Nucleoside diphosphate kinase from psychrophilic Pseudoalteromonas sp. AS-131 isolated from antarctic ocean (2015), Protein J., 34, 275-283.

Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment)	Organism
gene ndk, recombinant expression in Escherichia coli strain BL21Star(DE3)	Pseudoalteromonas sp.

Protein Variants

Protein Variants	Comment	Organism
D135A/E136T	site-directed mutagenesis	Pseudoalteromonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Pseudoalteromonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudoalteromonas sp.	-	isolated from antarctic ocean	-
Pseudoalteromonas sp. AS-131	-	isolated from antarctic ocean	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21Star(DE3) to homogeneity by ATP affinity chromatography and dialysis	Pseudoalteromonas sp.

Subunits

Subunits	Comment	Organism
dimer	tertiary structure homology modelling of wild-type and mutant enzymes using the tetrameric structure of mutagenized HaNDK, PDB ID 3VGU	Pseudoalteromonas sp.

Synonyms

Synonyms	Comment	Organism
ASNDK	-	Pseudoalteromonas sp.
nucleoside diphosphate kinase	-	Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	recombinant enzyme	Pseudoalteromonas sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	decrease in Kcat/Km with increasing temperature from 20°C to 37°C, inactivation at 50°C	Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	37	degeneration during recombinant expression at 37°C, decrease in Kcat/Km with increasing temperature from 20°C to 37°C, Tm is 38°C, inactivation of purified enzyme at 50°C	Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pseudoalteromonas sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)