Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.6 extracted from

  • Borthwick, L.A.; Kerbiriou, M.; Taylor, C.J.; Cozza, G.; Lascu, I.; Postel, E.H.; Cassidy, D.; Trouve, P.; Mehta, A.; Robson, L.; Muimo, R.
    Role of interaction and nucleoside diphosphate kinase B in regulation of the cystic fibrosis transmembrane conductance regulator function by cAMP-dependent protein kinase A (2016), PLoS ONE, 11, e0149097.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.6

Activating Compound

Activating Compound Comment Organism Structure
forskolin forskolin enhances NDPK-B interaction with CFTR 3fold in 16HBE14o- cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
apical membrane
-
 Homo sapiens
-
-
cytosol
-
 Homo sapiens 5829
-
additional information cAMP, acting through protein kinase A, regulates translocation of NDPK-B from the cytosol to the apical membrane leading to the formation of a functionally relevant complex between NDPK-B and CFTR Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens nucleoside diphosphate kinase B forms a functional complex with cell surface CFTR, binding analysis using peptide NDPK-B 36-54 (V36AMKFLRAS44EEHLKQHYID54), this region of NDPK-B, may constitute a key NDPK-B interaction domain for CFTR, the nucleotide binding domain 1 (NBD1, aa 351-727) of CFTR constitutes an NDPK-B interaction site with CFTR, surface plasmon resonance and NDPK-B and NBD1 protein-protein docking analysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22392 gene NME2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
16HBE14o- cell
-
 Homo sapiens
-
airway epithelium
-
 Homo sapiens
-
bronchial epithelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nucleoside diphosphate kinase B forms a functional complex with cell surface CFTR, binding analysis using peptide NDPK-B 36-54 (V36AMKFLRAS44EEHLKQHYID54), this region of NDPK-B, may constitute a key NDPK-B interaction domain for CFTR, the nucleotide binding domain 1 (NBD1, aa 351-727) of CFTR constitutes an NDPK-B interaction site with CFTR, surface plasmon resonance and NDPK-B and NBD1 protein-protein docking analysis Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
NDKB
-
 Homo sapiens
NDPK-B
-
 Homo sapiens
NM23-H2
-
 Homo sapiens
NME2
-
 Homo sapiens
nucleoside diphosphate kinase B
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function role of interaction and nucleoside diphosphate kinase B in regulation of the cystic fibrosis transmembrane conductance regulator function by cAMP-dependent protein kinase A, overview. Nucleoside diphosphate kinase B selectively forms a functional complex with CFTR, the nucleotide binding domain 1 (NBD1, aa 351-727) of CFTR constitutes an NDPK-B interaction site with CFTR, but NDPK-B associates only with cell surface CFTR. A functional interaction between CFTR, AMPKalpha1 and NDPK-A exists which is independent of NDPK-B. NDPK-B is important for the cAMP/PKA regulation of CFTR function Homo sapiens