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Literature summary for 2.7.4.6 extracted from

  • Haque, M.E.; Yoshida, Y.; Hasunuma, K.
    ROS resistance in Pisum sativum cv. Alaska: the involvement of nucleoside diphosphate kinase in oxidative stress responses via the regulation of antioxidants (2010), Planta, 232, 367-382.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Pisum sativum

Protein Variants

Protein Variants Comment Organism
E205K the mutation of isoform NDPK2 is associated with paraquat resistance Pisum sativum
I12L the mutation of isoform NDPK2 is associated with paraquat resistance Pisum sativum
P45S the mutation of isoform NDPK3 is associated with paraquat resistance Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16500
-
x * 16500, isoform NDPK3, calculated from amino acid sequence Pisum sativum
17600
-
x * 17600, low-molecular weight isoform NDPK2, calculated from amino acid sequence Pisum sativum
18500
-
x * 18500, high-molecular weight isoform NDPK2, calculated from amino acid sequence Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
paraquat-resistant line R3-1
-
Pisum sativum C0STY5 isoform NDPK2; cultivar Alaska
-
Pisum sativum C0STY7 isoform NDPK3; cultivar Alaska
-
Pisum sativum C0STY8 isoform NDPK3; paraquat-resistant line R3-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation Pisum sativum

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography, and gel filtration Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.3
-
wild type isoform NDPK2, using dTDP as substrate, at pH 7.5 and 22°C Pisum sativum
63.98
-
line R3-1 isoform NDPK3, using dGDP as substrate, at pH 7.5 and 22°C Pisum sativum
64.43
-
wild type isoform NDPK3, using dGDP as substrate, at pH 7.5 and 22°C Pisum sativum
90.55
-
line R3-1 isoform NDPK2, using dTDP as substrate, at pH 7.5 and 22°C Pisum sativum
103.5
-
line R3-1 isoform NDPK3, using dTDP as substrate, at pH 7.5 and 22°C Pisum sativum
112.6
-
wild type isoform NDPK3, using dTDP as substrate, at pH 7.5 and 22°C Pisum sativum
156.6
-
wild type isoform NDPK2, using dGDP as substrate, at pH 7.5 and 22°C Pisum sativum
283.9
-
line R3-1 isoform NDPK2, using dGDP as substrate, at pH 7.5 and 22°C Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dGDP dGDP is preferred over dTDP as an acceptor Pisum sativum ADP + dGTP
-
?
ATP + dTDP
-
Pisum sativum ADP + dTTP
-
?

Subunits

Subunits Comment Organism
? x * 16500, isoform NDPK3, calculated from amino acid sequence Pisum sativum
? x * 17600, low-molecular weight isoform NDPK2, calculated from amino acid sequence Pisum sativum
? x * 18500, high-molecular weight isoform NDPK2, calculated from amino acid sequence Pisum sativum

Synonyms

Synonyms Comment Organism
NDPK2 isoform Pisum sativum
NDPK3 isoform Pisum sativum
nucleoside diphosphate kinase
-
Pisum sativum

Expression

Organism Comment Expression
Pisum sativum isoform NDPK2 shows an increased level of activity and autophosphorylation in R3-1 plants compared to wild type plants up

General Information

General Information Comment Organism
physiological function there is a strong interaction between isoform NDPK2 and CAT1 in R3-1 plants, which possibly plays a vital role in the antioxidant defense against reactive oxygen species Pisum sativum