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Literature summary for 2.7.4.6 extracted from
- Crystal structure of the Bacillus anthracis nucleoside diphosphate kinase and its characterization reveals an enzyme adapted to perform under stress conditions (2009), Proteins, 76, 496-506.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bacillus anthracis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G19V | the mutation rigidifies the 43-63 loop in Ndk, using GTP as the phosphate donor a 300fold reduction in phosphotransferase activity is observed | Bacillus anthracis |
| H115Q | H115Q is incompetent in autophosphorylation, confirming the role of histidine as a phosphate acceptor | Bacillus anthracis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition at 10 mM | Bacillus anthracis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the activity is dependent on a metal ion (mainly Mg2+) | Bacillus anthracis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NDP | Bacillus anthracis | - |
ADP + NTP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | Q81SV8 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the presence of diethyl dicarbonate inhibits the autophosphorylation of Ndk | Bacillus anthracis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GDP | - |
Bacillus anthracis | ADP + GTP | - |
? | |
| ATP + NDP | - |
Bacillus anthracis | ADP + NTP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | exists as a hexamer that is composed of two SDS-stable trimers interacting in a back-to-back association | Bacillus anthracis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDK | - |
Bacillus anthracis |
| nucleoside diphosphate kinase | - |
Bacillus anthracis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 95 | - |
Bacillus anthracis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10.5 | - |
Bacillus anthracis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus anthracis | |
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