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Literature summary for 2.7.4.6 extracted from
- Trypanosoma cruzi: multiple nucleoside diphosphate kinase isoforms in a single cell (2008), Exp. Parasitol., 120, 103-107.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
dTDP | pH 7.5, 35°C, isozyme NDPK2 | Trypanosoma cruzi strain CL Brener |  |
| 0.86 | - |
dTDP | pH 7.5, 35°C, isozyme NDPK1 | Trypanosoma cruzi strain CL Brener | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | mainly | Trypanosoma cruzi strain CL Brener | 5829 | - |
| mitochondrion | slightly | Trypanosoma cruzi strain CL Brener | 5739 | - |
| additional information | subcellular localization analysis, overview | Trypanosoma cruzi strain CL Brener | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Trypanosoma cruzi strain CL Brener | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dTDP | Trypanosoma cruzi strain CL Brener | - |
ADP + dTTP | - |
r | |
| additional information | Trypanosoma cruzi strain CL Brener | kinetic parameters and regulation mechanisms of NDPK1 and NDPK2, overview | ? | - |
? | |
| NTP + NDP | Trypanosoma cruzi strain CL Brener | - |
NDP + NTP | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi strain CL Brener | Q4E256 | multiple enzyme isoforms | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma cruzi strain CL Brener | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dTDP | - |
Trypanosoma cruzi strain CL Brener | ADP + dTTP | - |
r | |
| additional information | kinetic parameters and regulation mechanisms of NDPK1 and NDPK2, overview | Trypanosoma cruzi strain CL Brener | ? | - |
? | |
| NTP + NDP | - |
Trypanosoma cruzi strain CL Brener | NDP + NTP | - |
r | |
| NTP + NDP | the high-energy phosphate is mainly supplied by ATP, but the enzymes have broad substrate specificity and use the ribose and deoxyribose forms of both, purine and pyrimidine nucleotides, ping-pong mechanism with a phosphoenzyme/phosphohistidine intermediary involved in the transfer reaction, overview | Trypanosoma cruzi strain CL Brener | NDP + NTP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | NDPK1 is a short enzyme isoform, whereas NDPK2 is a long one containing a DM10 motif | Trypanosoma cruzi strain CL Brener |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDPK | - |
Trypanosoma cruzi strain CL Brener |
| nucleoside diphosphate kinase | - |
Trypanosoma cruzi strain CL Brener |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Trypanosoma cruzi strain CL Brener |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Trypanosoma cruzi strain CL Brener |
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Release 2023.1 (January 2023)
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