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Literature summary for 2.7.4.6 extracted from

  • Hammargren, J.; Salinas, T.; Marechal-Drouard, L.; Knorpp, C.
    The pea mitochondrial nucleoside diphosphate kinase cleaves DNA and RNA (2007), FEBS Lett., 581, 3507-3511.
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
isozyme NDPK3, expression in Escherichia coli Pisum sativum

Protein Variants

Protein Variants Comment Organism
A117D complete loss of nucleoside diphosphate kinase activity, but no loss of nuclease activity Pisum sativum
S69A complete loss of nucleoside diphosphate kinase activity, but no loss of nuclease activity Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibition of nuclease activity Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion intermembrane space Pisum sativum 5739
-
mitochondrion intermembrane space of mitochondria Pisum sativum 5739
-

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 recombinant isoform Ndpk3, enzyme additionally has nuclease activity cleaving supercoiled plasmid DNA, tRNAs or the 3’UTR of atp9 mRNA
-
Pisum sativum
-
 isozyme NDPK3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally has nuclease activity cleaving supercoiled plasmid DNA, tRNAs or the 3’UTR of atp9 mRNA. Nuclease- and kinase mechanisms are separate Pisum sativum ?
-
 ?
additional information NDPK3 protein additionally cleaves not only supercoiled plasmid DNA, but also highly structured RNA molecules such as tRNAs or the 3'UTR of the atp9 mRNA suggesting that the NDPK3 nuclease activity has a structural requirement, ATP inhibits this nuclease activity, while ADP has no effect, the nuclease- and the kinase-mechanisms are separate, overview Pisum sativum ?
-
 ?

Synonyms

Synonyms Comment Organism
NDPK
-
 Pisum sativum
nucleoside diphosphate kinase
-
 Pisum sativum