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Literature summary for 2.7.4.6 extracted from
- Nucleoside-triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 1.Isolation of the crystalline enzyme from brewers´yeast (1964), J. Biol. Chem., 239, 301-309.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces pastorianus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NDP + NTP | Saccharomyces pastorianus | - |
NTP + NDP | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces pastorianus | - |
brewer's yeast, Anheuser-Busch strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces pastorianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1600 | - |
25°C | Saccharomyces pastorianus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, crude acetone powder, several months | Saccharomyces pastorianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + ADP | - |
Saccharomyces pastorianus | ADP + ATP | - |
? | |
| ATP + CDP | - |
Saccharomyces pastorianus | ADP + CTP | - |
r | |
| ATP + dADP | - |
Saccharomyces pastorianus | ADP + dATP | - |
r | |
| ATP + dCDP | - |
Saccharomyces pastorianus | ADP + dCTP | - |
r | |
| ATP + dGDP | - |
Saccharomyces pastorianus | ADP + dGTP | - |
r | |
| ATP + UDP | - |
Saccharomyces pastorianus | ADP + UTP | - |
? | |
| dATP + CDP | - |
Saccharomyces pastorianus | dADP + CTP | - |
r | |
| dATP + GDP | - |
Saccharomyces pastorianus | dADP + GTP | - |
? | |
| dATP + IDP | - |
Saccharomyces pastorianus | dADP + ITP | - |
? | |
| dATP + UDP | - |
Saccharomyces pastorianus | dADP + UTP | - |
? | |
| GTP + ADP | - |
Saccharomyces pastorianus | GDP + ATP | - |
r | |
| ITP + ADP | - |
Saccharomyces pastorianus | IDP + ATP | - |
? | |
| NDP + NTP | - |
Saccharomyces pastorianus | NTP + NDP | - |
r | |
| NTP + nucleoside diphosphate | broad specificity | Saccharomyces pastorianus | NDP + NTP | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces pastorianus |
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Release 2023.1 (January 2023)
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