KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
GTP | cosubstrate dTDP, 30°C, pH 8.0 | Avena sativa | |
0.19 | - |
ATP | cosubstrate dTDP, 30°C, pH 8.0 | Avena sativa | |
0.21 | 0.39 | dTDP | 30°C, pH 8.0 | Avena sativa | |
0.26 | 0.37 | CDP | 30°C, pH 8.0 | Avena sativa | |
0.26 | 0.37 | CDP | cosubstrate ATP | Avena sativa | |
0.42 | - |
dCDP | cosubstrate ATP, 30°C, pH 8.0 | Avena sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
N-terminal sequence homology of kinases from different organisms | Avena sativa |
18000 | - |
6 * 18000, SDS-PAGE | Avena sativa |
100000 | - |
gel filtration | Avena sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP + NTP | Avena sativa | may be involved in regulation of growth, development and signal-transduction processes | NTP + NDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | - |
oat, cv. Garry oats | - |
Purification (Comment) | Organism |
---|---|
- |
Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | etiolated | Avena sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1736 | - |
30°C, pH 8.0 | Avena sativa |
Storage Stability | Organism |
---|---|
-70°C, several months | Avena sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CDP | - |
Avena sativa | ADP + CTP | - |
? | |
ATP + dCDP | - |
Avena sativa | ADP + dCTP | - |
? | |
ATP + dTDP | in decreasing order of activity: UDP, dUDP, dCDP, GDP, dGDP, CDP | Avena sativa | ADP + dTTP | - |
? | |
ATP + dTDP | GTP can fully replace ATP | Avena sativa | ADP + dTTP | - |
? | |
ATP + dTDP | best substrate: dTDP | Avena sativa | ADP + dTTP | - |
? | |
GTP + GDP | in decreasing order of activity: UTP, dGTP, dTTP, CTP, dCTP, dUTP | Avena sativa | GDP + GTP | - |
? | |
GTP + GDP | equally active as ATP | Avena sativa | GDP + GTP | - |
? | |
NDP + NTP | may be involved in regulation of growth, development and signal-transduction processes | Avena sativa | NTP + NDP | - |
? | |
NTP + nucleoside diphosphate | broad specificity | Avena sativa | NDP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 18000, SDS-PAGE | Avena sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Avena sativa |