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Literature summary for 2.7.4.6 extracted from
- Nucleoside diphosphate kinase from Xenopus oocytes; partial purification and characterization (1990), Biochim. Biophys. Acta, 1041, 296-304.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | kinetics, enhanced by Zn2+ | Xenopus laevis |  |
| additional information | no inhibition by Zn2+ alone | Xenopus laevis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | predominant | Xenopus laevis | 5829 | - |
| particle-bound | - |
Xenopus laevis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | probably tightly bound, 30% of activity even after prolonged exposure to EDTA | Xenopus laevis | |
| Zn2+ | not | Xenopus laevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21500 | - |
x * 21500, SDS-PAGE | Xenopus laevis |
| 45000 | - |
dimer, gel filtration | Xenopus laevis |
| 70000 | - |
gel filtration | Xenopus laevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xenopus laevis | - |
female mature frog | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, cytosolic enzyme | Xenopus laevis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | the catalytic mechanism involves a phosphoenzyme intermediate | Xenopus laevis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oocyte | - |
Xenopus laevis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 125 | - |
- |
Xenopus laevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + CDP | - |
Xenopus laevis | ADP + CTP | - |
? | |
| ATP + UDP | - |
Xenopus laevis | ADP + UTP | - |
? | |
| CTP + CDP | - |
Xenopus laevis | CDP + CTP | - |
? | |
| gamma-S-ATP + GDP | poor substrate | Xenopus laevis | ADP + gamma-S-GTP | - |
? | |
| gamma-S-GTP + GDP | poor substrate | Xenopus laevis | GDP + gamma-S-GTP | - |
? | |
| GTP + ADP | - |
Xenopus laevis | GDP + ATP | - |
r | |
| GTP + CDP | - |
Xenopus laevis | GDP + CTP | - |
r | |
| GTP + GDP | - |
Xenopus laevis | GDP + GTP | - |
? | |
| GTP + UDP | - |
Xenopus laevis | GDP + UTP | - |
? | |
| NTP + nucleoside diphosphate | broad specificity | Xenopus laevis | NDP + NTP | - |
? | |
| NTP + nucleoside diphosphate | many ribo- and deoxyribonucleoside triphosphates act as donors | Xenopus laevis | NDP + NTP | - |
? | |
| NTP + nucleoside diphosphate | transfers gamma-phosphate from NTP to any nucleoside diphosphate | Xenopus laevis | NDP + NTP | - |
? | |
| UTP + CDP | - |
Xenopus laevis | UDP + CTP | - |
? | |
| UTP + dCDP | - |
Xenopus laevis | UDP + dCTP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21500, SDS-PAGE | Xenopus laevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Xenopus laevis |
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