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Literature summary for 2.7.4.6 extracted from
- Nucleoside diphosphate kinase from brain. Purification and effect on microtubule assembly in vitro (1984), Eur. J. Biochem., 144, 233-241.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
6 * 17000, SDS-PAGE | Sus scrofa |
| 100000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tubulin-GDP-complex + ATP | Sus scrofa | - |
tubulin-GTP-complex + ADP | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| several molecular species can be separated by electrophoresis or chromatofocusing | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2026 | - |
25°C | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tubulin-GDP | - |
Sus scrofa | ADP + tubulin-GTP | - |
? | |
| GTP + ADP | - |
Sus scrofa | GDP + ATP | - |
r | |
| NTP + nucleoside diphosphate | - |
Sus scrofa | NDP + NTP | - |
? | |
| tubulin-GDP-complex + ATP | - |
Sus scrofa | tubulin-GTP-complex + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 17000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sus scrofa |
| 37 | - |
assay with tubulin-GDP as substrate | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 67 | - |
10 min, crude, stable in 0.9 M ammonium sulfate at 22% saturation | Sus scrofa |
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Release 2023.1 (January 2023)
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