Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + [phosphate]n | Pseudomonas aeruginosa | best substrate | ADP + [phosphate]n-1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, separation from polyphosphate kinase activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + (phosphate)n | the activity originates from combined action of the polyphosphate:ADP phosphotransferase and adenylate kinase | Pseudomonas aeruginosa | ADP + (phosphate)n-1 | - |
? | |
AMP + [phosphate]n | best substrate | Pseudomonas aeruginosa | ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | best substrate is [phosphate]15 | Pseudomonas aeruginosa | ADP + [phosphate]n-1 | - |
? | |
additional information | the partially purified polyphosphate:ADP phosphotransferase is independent of polyphosphate kinase, PPK, and can function as polyphosphate-dependent nucleoside diphosphate kinase, PADP, which most prefers GDP to the other three nucleoside diphosphates as a phospho-acceptor. Both of the polyphosphate-utilizing activities require short polyphosphate as a phospho-donor whose chain length is below 75. The PAP activity mainly originates from the combined action of adenylate kinase, ADK, and the PADP independent of PPK. Each partially purified enzyme alone cannot mediate phosphorylation of AMP with polyphosphate, but coincubation of the both enzymes results in a marked expression of PAP activity, confirming that PADP and ADK constitute the PAP | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PADP | - |
Pseudomonas aeruginosa |
PAP | - |
Pseudomonas aeruginosa |
polyphosphate:ADP phosphotransferase | - |
Pseudomonas aeruginosa |
polyphosphate:AMP phosphotransferase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
additional information | overexpression in strain PAO1 leads to a massive production of polyphosphate kinase, PPK, and a marked enhancement of polyphosphate-synthesizing activity, while the PAP activity is not affected by overproduction of PPK | Pseudomonas aeruginosa |