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Literature summary for 2.7.4.33 extracted from

  • Resnick, S.M.; Zehnder, A.J.B.
    In vitro ATP regeneration from polyphosphate and AMP by polyphosphate:AMP phosphotransferase and adenylate kinase from Acinetobacter johnsonii 210A (2000), Appl. Environ. Microbiol., 66, 2045-2051.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.33

Application

Application Comment Organism
synthesis PPT catalyzes a key reaction in the cell-free regeneration of ATP from AMP and polyphosphate. The PPT/AdK system provides an alternative to existing enzymatic ATP regeneration systems in which phosphoenolpyruvate and acetylphosphate serve as phosphoryl donors and has the advantage that AMP and polyP are stable, inexpensive substrates Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + [phosphate]n Acinetobacter johnsonii
-
 ADP + [phosphate]n-1
-
 ?
AMP + [phosphate]n Acinetobacter johnsonii 210A
-
 ADP + [phosphate]n-1
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-
Acinetobacter johnsonii 210A
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-dAMP + (phosphate)n
-
 Acinetobacter johnsonii 2'-dADP + (phosphate)n-1
-
 ?
2'-dAMP + (phosphate)n
-
 Acinetobacter johnsonii 210A 2'-dADP + (phosphate)n-1
-
 ?
2'-deoxy-AMP + [phosphate]n
-
 Acinetobacter johnsonii 2'-deoxy-ADP + [phosphate]n-1
-
 ?
AMP + (phosphate)n
-
 Acinetobacter johnsonii ADP + (phosphate)n-1
-
 ?
AMP + (phosphate)n
-
 Acinetobacter johnsonii 210A ADP + (phosphate)n-1
-
 ?
AMP + [phosphate]n
-
 Acinetobacter johnsonii ADP + [phosphate]n-1
-
 ?
AMP + [phosphate]n
-
 Acinetobacter johnsonii 210A ADP + [phosphate]n-1
-
 ?
additional information GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates Acinetobacter johnsonii ?
-
 ?
additional information PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates Acinetobacter johnsonii ?
-
 ?
additional information GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates Acinetobacter johnsonii 210A ?
-
 ?
additional information PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates Acinetobacter johnsonii 210A ?
-
 ?

Synonyms

Synonyms Comment Organism
polyphosphate:AMP phosphotransferase
-
 Acinetobacter johnsonii
PPT
-
 Acinetobacter johnsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Acinetobacter johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Acinetobacter johnsonii

General Information

General Information Comment Organism
additional information ATP regeneration from AMP and polyphophate using PPT, firefly luciferase and hexokinase as model ATP-requiring enzymes, ADP can be converted to ATP by adenylate kinase, AdK. Establishment of a PPT/AdK system for ATP regeneration with coupled hexokinase, overview Acinetobacter johnsonii
physiological function PPT from Acinetobacter johnsonii is specific for AMP and 2'-dAMP Acinetobacter johnsonii