Application | Comment | Organism |
---|---|---|
synthesis | design of an ATP regeneration system with poylphosphate-AMP phosphotransferase and polyphosphate kinase. The ATP regeneration system can also be used as a GTP regeneration system | Myxococcus xanthus |
synthesis | the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA | Myxococcus xanthus |
synthesis | the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA | Acinetobacter johnsonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Myxococcus xanthus | 16020 | - |
soluble | - |
Acinetobacter johnsonii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Myxococcus xanthus | |
Mg2+ | required | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + [phosphate]n | Myxococcus xanthus | - |
ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | Acinetobacter johnsonii | - |
ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | Myxococcus xanthus DK101 | - |
ADP + [phosphate]n-1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Myxococcus xanthus | - |
- |
- |
Myxococcus xanthus DK101 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 200fold by membrane fraction preparation and washing with detergent Triton X-100 and SB-12 | Myxococcus xanthus |
partial | Myxococcus xanthus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
partially purified native enzyme, pH 8.0, 37°C, ADP synthesis | Myxococcus xanthus |
Storage Stability | Organism |
---|---|
4°C, membrane fraction, over 2 months, stable | Myxococcus xanthus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + (phosphate)n | - |
Myxococcus xanthus | ADP + (phosphate)n-1 | - |
? | |
AMP + (phosphate)n | - |
Myxococcus xanthus DK101 | ADP + (phosphate)n-1 | - |
? | |
AMP + [phosphate]n | - |
Myxococcus xanthus | ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | - |
Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | - |
Myxococcus xanthus DK101 | ADP + [phosphate]n-1 | - |
? | |
GMP + [phosphate]n | - |
Myxococcus xanthus | GDP + [phosphate]n-1 | - |
? | |
GMP + [phosphate]n | - |
Acinetobacter johnsonii | GDP + [phosphate]n-1 | - |
? | |
GMP + [phosphate]n | - |
Myxococcus xanthus DK101 | GDP + [phosphate]n-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Myxococcus xanthus |
PAP | - |
Acinetobacter johnsonii |
poylphosphate-AMP phosphotransferase | - |
Myxococcus xanthus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Myxococcus xanthus |
37 | - |
assay at | Acinetobacter johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Myxococcus xanthus |
8 | - |
assay at | Acinetobacter johnsonii |