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Literature summary for 2.7.4.31 extracted from

  • Wang, Y; Xu, H; Jones, MK; White, R.H.
    Identification of the final two genes functioning in methanofuran biosynthesis in Methanocaldococcus jannaschii (2015), J. Bacteriol., 197, 2850-2858.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.31

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete loss of activity Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MfnE exclusively uses Mg2+ as a cofactor to facilitate phosphoryl group transfer Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, SDS-PAGE Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57900
-
-
Methanocaldococcus jannaschii DSM 2661 Q57900
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.072
-
 substrate AMP, pH 7.0, 80°C Methanocaldococcus jannaschii
0.16
-
 substrate 5-(aminomethyl)-3-furanmethanol-phosphate, pH 7.0, 80°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + ATP
-
 Methanocaldococcus jannaschii 2 ADP
-
 ?
AMP + ATP reaction of adenylate kinase, EC 2.7.4.3 Methanocaldococcus jannaschii 2 ADP
-
 ?
AMP + ATP
-
 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 ?
AMP + ATP reaction of adenylate kinase, EC 2.7.4.3 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 ?
ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
-
 Methanocaldococcus jannaschii ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
-
 ?
ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
-
 Methanocaldococcus jannaschii DSM 2661 ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
MJ0458
-
 Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function enzyme catalyzes the formation of methanofuran precursor 5-(aminomethyl)-3-furanmethanol-diphosphate (F1-PP) from ATP Methanocaldococcus jannaschii