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Literature summary for 2.7.4.3 extracted from

  • Thach, T.T.; Lee, S.
    New crystal structures of adenylate kinase from Streptococcus pneumoniae D39 in two conformations (2014), Acta Crystallogr. Sect. F, 70, 1468-1471.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene adk, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in ligand-free and inhibitor Ap5A-bound states, hanging drop vapour diffusion method, mixing of 0.0025 ml of 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 50-150 mM NaCl, 5 mM MgCl2, with 0.0025 ml reservoir solution containing 1.0 M sodium citrate, 0.1 M HEPES, pH 7.5 for the ligand-free crystals and 0.2 M sodium acetate, pH 7.2, 30% PEG 8000 for the inhibitor-bound crystals, 22°C, X-ray diffraction structure determination and analysis at 1.96 and 1.65 A resolution, respectively. SpAdK can crystallize promiscuously in different forms, and the open structure is flexible in conformation Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
P1,P5-bis(adenosine-5'-)pentaphosphate i.e. Ap5A, a two substrate-mimicking inhibitor, binding affinity of SpAdK to Ap5A is determined by isothermal titration calorimetry Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
-
Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + AMP Streptococcus pneumoniae
-
2 ADP
-
r

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q04ML5 gene adk
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration, the GST moiety is cleaved using tobacco etch virus protease Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
Streptococcus pneumoniae 2 ADP
-
r

Subunits

Subunits Comment Organism
? x * 24000, detagged recombinant enzyme, SDS-PAGE, x * 23721, sequence calculation Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
ADK
-
Streptococcus pneumoniae

General Information

General Information Comment Organism
additional information the ligand-free enzyme structure reveals an open conformation Streptococcus pneumoniae
physiological function adenylate kinases play a critical role in intercellular homeostasis by the interconversion of ATP and AMP to two ADP molecules Streptococcus pneumoniae