Literature summary for 2.7.4.3 extracted from

Mukhopadhyay, A.; Kladova, A.V.; Bursakov, S.A.; Gavel, O.Y.; Calvete, J.J.; Shnyrov, V.L.; Moura, I.; Moura, J.J.; Romao, M.J.; Trincao, J.
Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria (2011), J. Biol. Inorg. Chem., 16, 51-61.

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Cloned(Commentary)

Cloned (Comment)	Organism
the cobalt- or iron-bound enzyme is expressed in Escherichia coli BL21(DE3) cells	Megalodesulfovibrio gigas

Crystallization (Commentary)

Crystallization (Comment)	Organism
adenylate kinase bound to Zn2+, Co2+ or Fe2+, hanging drop vapor diffusion method, using 0.2 M sodium/potassium tartrate, 0.1 M 2-(N-morpholino)ethanesulfonic acid (pH 6.5), and 20% (w/v) PEG 200 or PEG 800	Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	adenylate kinase contains a bivalent metal ion (zinc, cobalt, or iron)	Megalodesulfovibrio gigas
Fe2+	adenylate kinase contains a bivalent metal ion (zinc, cobalt, or iron)	Megalodesulfovibrio gigas
Zn2+	adenylate kinase contains a bivalent metal ion (zinc, cobalt, or iron)	Megalodesulfovibrio gigas

Organism

Organism	UniProt	Comment	Textmining
Megalodesulfovibrio gigas	C7U112	-	-

Purification (Commentary)

Purification (Comment)	Organism
Blue Sepharose column chromatography and Superdex 75 gel filtration	Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + AMP	-	Megalodesulfovibrio gigas	2 ADP	-	?

Subunits

Subunits	Comment	Organism
dimer	cobalt-bound enzyme, X-ray crystallography	Megalodesulfovibrio gigas
monomer	iron- or zinc-bound enzyme, X-ray crystallography	Megalodesulfovibrio gigas

Synonyms

Synonyms	Comment	Organism
ATP:AMP phosphotransferase	-	Megalodesulfovibrio gigas

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Release 2023.1 (January 2023)