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Literature summary for 2.7.4.3 extracted from

  • Davlieva, M.; Shamoo, Y.
    Crystal structure of a trimeric archaeal adenylate kinase from the mesophile Methanococcus maripaludis with an unusually broad functional range and thermal stability (2010), Proteins, 78, 357-364.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Methanococcus maripaludis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using in 3.4 M ammonium chloride, 0.1 M sodium acetate (pH 4.7), and 3% ethylene glycol (v/v), at 22°C Methanococcus maripaludis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00537
-
 ATP at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.00959
-
 AMP at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.01659
-
 ATP at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.0372
-
 AMP at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.05547
-
 AMP at 60°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.07389
-
 AMP at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.09471
-
 ATP at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
0.1349
-
 ATP at 60°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis Q6LYG0 strain ATCC 43000D
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q-XL Sepharose column chromatography, Affi-Gel blue resin colum chromatography, and Superdex-200 gel filtration Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Methanococcus maripaludis ADP + ADP
-
 ?

Subunits

Subunits Comment Organism
trimer x-ray crystallography Methanococcus maripaludis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 excellent activity over a broad temperature range of 30-60°C Methanococcus maripaludis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
74
-
 the melting temperature is at 74°C Methanococcus maripaludis