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Literature summary for 2.7.4.3 extracted from

  • Panayiotou, C.; Solaroli, N.; Johansson, M.; Karlsson, A.
    Evidence of an intact N-terminal translocation sequence of human mitochondrial adenylate kinase 4 (2010), Int. J. Biochem. Cell Biol., 42, 62-69.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K4G when the amino acid residue is mutated, the protein is imported in mitochondria Homo sapiens
K4G/R7G when both amino acid residues are mutated simultaneously the protein remains in the cytosol Homo sapiens
R7G when the amino acid residue is mutated, the protein is imported in mitochondria Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
AMP recombinant adenylate kinase 4, using GTP as cosubstrate Homo sapiens
0.0053
-
AMP recombinant adenylate kinase 4, using ATP as cosubstrate Homo sapiens
0.507
-
dAMP recombinant adenylate kinase 4, using ATP as cosubstrate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE, fused with the GST tag the recombinant protein has a size of about 53000 Da Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27144
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP adenylate kinase 4 shows slightly lower efficiency for the phosphorylation of AMP with ATP compared to the phosphorylation of AMP with GTP Homo sapiens ADP + ADP
-
?
ATP + CMP phosphorylation of CMP is also detected but to a lesser extend Homo sapiens ADP + CDP
-
?
ATP + dAMP dAMP is the poorest substrate Homo sapiens ADP + dADP
-
?
GTP + AMP isozyme adenylate kinase 4 shows its highest efficiency when phosphorylating AMP with GTP, when GTP is used as phosphate donor only AMP is clearly phosphorylated and the phosphorylation efficiency for dAMP, CMP and dCMP is very low Homo sapiens GDP + ADP
-
?
additional information adenylate kinase 4 catalyzes the phosphorylation of AMP, dAMP,CMPand dCMP with ATP or GTP as phosphate donors and also phosphorylates AMP with UTP as phosphate donor Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
adenylate kinase 4
-
Homo sapiens
AK4 isozyme Homo sapiens