Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K4G | when the amino acid residue is mutated, the protein is imported in mitochondria | Homo sapiens |
K4G/R7G | when both amino acid residues are mutated simultaneously the protein remains in the cytosol | Homo sapiens |
R7G | when the amino acid residue is mutated, the protein is imported in mitochondria | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
AMP | recombinant adenylate kinase 4, using GTP as cosubstrate | Homo sapiens | |
0.0053 | - |
AMP | recombinant adenylate kinase 4, using ATP as cosubstrate | Homo sapiens | |
0.507 | - |
dAMP | recombinant adenylate kinase 4, using ATP as cosubstrate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE, fused with the GST tag the recombinant protein has a size of about 53000 Da | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27144 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | adenylate kinase 4 shows slightly lower efficiency for the phosphorylation of AMP with ATP compared to the phosphorylation of AMP with GTP | Homo sapiens | ADP + ADP | - |
? | |
ATP + CMP | phosphorylation of CMP is also detected but to a lesser extend | Homo sapiens | ADP + CDP | - |
? | |
ATP + dAMP | dAMP is the poorest substrate | Homo sapiens | ADP + dADP | - |
? | |
GTP + AMP | isozyme adenylate kinase 4 shows its highest efficiency when phosphorylating AMP with GTP, when GTP is used as phosphate donor only AMP is clearly phosphorylated and the phosphorylation efficiency for dAMP, CMP and dCMP is very low | Homo sapiens | GDP + ADP | - |
? | |
additional information | adenylate kinase 4 catalyzes the phosphorylation of AMP, dAMP,CMPand dCMP with ATP or GTP as phosphate donors and also phosphorylates AMP with UTP as phosphate donor | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylate kinase 4 | - |
Homo sapiens |
AK4 | isozyme | Homo sapiens |