Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.172 | - |
AMP | adenylate kinase 5 domain AK5p1, with ATP as phosphate donor | Homo sapiens | |
2 | - |
dAMP | Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y6K8 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | adenylate kinase 5 domain AK5p1, at the assay conditions used, and at lower concentrations of substrate, AK5p1 shows generally a higher affinity for AMP compared to dAMP | Homo sapiens | ADP + ADP | - |
? | |
ATP + CMP | adenylate kinase 5 domain AK5p1, the relative efficiency of CMP is about 15% compared to AMP | Homo sapiens | ADP + CDP | - |
? | |
ATP + dAMP | adenylate kinase 5 domain AK5p1 | Homo sapiens | ADP + dADP | - |
? | |
ATP + dCMP | adenylate kinase 5 domain AK5p1, the relative efficiency of dCMP is about 15% compared to AMP | Homo sapiens | ADP + dCDP | - |
? | |
additional information | AK5p1 phosphorylates AMP, CMP, dAMP and dCMP with ATP or GTP as phosphate donors, AK5p2 phosphorylates AMP, CMP and dAMP when ATP is used as phosphate donor and AMP, CMP and dCMP with GTP as phosphate donor, AK5p2 cannot phosphorylate dAMP in the presence of GTP | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylate kinase 5 | isozyme, human AK5 has two separate functional domains both having enzymatic activity | Homo sapiens |
AK5 | isozyme | Homo sapiens |
AK5p1 | domain of adenylate kinase 5, human AK5 has two separate functional domains both having enzymatic activity | Homo sapiens |
AK5p2 | domain of adenylate kinase 5, human AK5 has two separate functional domains both having enzymatic activity | Homo sapiens |