Literature summary for 2.7.4.3 extracted from

Kladova, A.V.; Gavel, O.Y.; Mukhopaadhyay, A.; Boer, D.R.; Teixeira, S.; Shnyrov, V.L.; Moura, I.; Moura, J.J.; Romao, M.J.; Trincao, J.; Bursakov, S.A.
Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: purification, crystallization and preliminary X-ray diffraction analysis (2009), Acta Crystallogr. Sect. F, 65, 926-929.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Megalodesulfovibrio gigas

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme in complex with Zn2+, recombinant enzymes in complex with Fe2+ or Co2+, hanging drop vapor diffusion method, using 0.2 M sodium/potassium tartrate, 0.1 M MES pH 6.5 and 20% (w/v) PEG 8K	Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the native enzyme contains 0.03 mol zinc per mole of protein	Megalodesulfovibrio gigas

Organism

Organism	UniProt	Comment	Textmining
Megalodesulfovibrio gigas	C7U112	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Blue Sepharose column chromatography, and Superdex 75 gel filtration	Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + AMP	-	Megalodesulfovibrio gigas	ADP + ADP	-	r

Synonyms

Synonyms	Comment	Organism
ATP:AMP phosphotransferase	-	Megalodesulfovibrio gigas

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Release 2023.1 (January 2023)