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Literature summary for 2.7.4.3 extracted from

  • Davlieva, M.; Shamoo, Y.
    Structure and biochemical characterization of an adenylate kinase originating from the psychrophilic organism Marinibacillus marinus (2009), Acta Crystallogr. Sect. F, 65, 751-756.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Jeotgalibacillus marinus

Crystallization (Commentary)

Crystallization (Comment) Organism
with the inhibitor P1,P5-di(adenosine-5')-pentaphosphate bound to the active site, sitting drop vapor diffusion method, using 1.5 M sodium citrate pH 6.5, 150 mM sodium chloride, 0.5% n-dodecyl-N,N-dimethylamine-N-oxide, at 10°C Jeotgalibacillus marinus

Inhibitors

Inhibitors Comment Organism Structure
P1,P5-di(adenosine-5')pentaphosphate
-
 Jeotgalibacillus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 ATP at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 6 5mM KCl Jeotgalibacillus marinus
0.002
-
 ATP at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl Jeotgalibacillus marinus
0.002
-
 ATP at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl Jeotgalibacillus marinus
0.0214
-
 AMP at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl Jeotgalibacillus marinus
0.0216
-
 AMP at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl Jeotgalibacillus marinus
0.024
-
 AMP at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl Jeotgalibacillus marinus

Organism

Organism UniProt Comment Textmining
Jeotgalibacillus marinus Q6B341 strain ATCC 29841
-

Purification (Commentary)

Purification (Comment) Organism
Q-XL Sepharose column chromatography, Affi-Gel Blue resin column chromatography, and Superdex-200 gel filtration Jeotgalibacillus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Jeotgalibacillus marinus ADP + ADP
-
 ?