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Literature summary for 2.7.4.3 extracted from
- Three extremely thermostable proteins from Sulfolobuc and a reappraisal of the 'traffic rules' (1996), Biol. Chem., 377, 505-512.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Thermolysin | significant increase of specific activity (1.5-fold) after a short (60 min, 50 °C) proteolytic treatment with thermolysin | Sulfolobus acidocaldarius |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced in Escherichia coli | Sulfolobus acidocaldarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
ATP | 90°C, pH is not specified in the publication | Sulfolobus acidocaldarius |  |
| 1.1 | - |
AMP | 90°C, pH is not specified in the publication | Sulfolobus acidocaldarius | |
| 1.3 | - |
ADP | 90°C, pH is not specified in the publication | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21170 | - |
2 * 21170, calculated from sequence | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | P35028 | - |
- |
| Sulfolobus acidocaldarius DSM 639 | P35028 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Sulfolobus acidocaldarius | 2 ADP | - |
r | |
| ATP + AMP | - |
Sulfolobus acidocaldarius DSM 639 | 2 ADP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 21170, calculated from sequence | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
long term incubation | Sulfolobus acidocaldarius |
| 90 | - |
incubation time 60 s | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
above 24 h | Sulfolobus acidocaldarius |
| 95 | - |
Tm-value, transition is not complete at 100°C | Sulfolobus acidocaldarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 170 | - |
ATP | 70°C, pH is not specified in the publication | Sulfolobus acidocaldarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
ATP formation | Sulfolobus acidocaldarius |
| 6 | - |
ADP formation | Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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