Application | Comment | Organism |
---|---|---|
additional information | CFTR gating mechanism based on adenylate kinase activity | Homo sapiens |
additional information | CFTR gating mechanism based on adenylate kinase activity | Mus musculus |
Cloned (Comment) | Organism |
---|---|
cloned into vector pET28b and expressed in Escherichia coli BL21DE3pLys cells | Homo sapiens |
cloned into vector pET28b and expressed in Escherichia coli BL21DE3pLys cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
G551D | mutation affects the ability of the nucleotide-binding domain 1 of CFTR to dimerize, has an exaggerated nonlinear phase compared with the wild type | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diadenosine polyphosphate | inhibits adenylate kinase activity of the nucleotide-binding domains 1 and 2 of CFTR | Homo sapiens | |
diadenosine polyphosphate | inhibits adenylate kinase activity of the nucleotide-binding domain 1 of CFTR | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ATP | wild type | Mus musculus | |
0.03 | - |
AMP | wild type | Mus musculus | |
0.04 | - |
AMP | nucleotide-binding domain 2 of wild type | Homo sapiens | |
0.06 | - |
AMP | nucleotide-binding domain 1 of wild type | Homo sapiens | |
0.07 | - |
AMP | G551D mutant | Mus musculus | |
0.07 | - |
ATP | nucleotide-binding domain 2 of wild type | Homo sapiens | |
0.08 | - |
ATP | G551D mutant | Mus musculus | |
0.08 | - |
ATP | nucleotide-binding domain 1 of wild type | Homo sapiens | |
0.28 | - |
ADP | nucleotide-binding domain 2 of wild type | Homo sapiens | |
0.3 | - |
ADP | nucleotide-binding domain 1 of wild type | Homo sapiens | |
0.36 | - |
ADP | G551D mutant | Mus musculus | |
0.45 | - |
ADP | wild type | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P13569 | - |
- |
Mus musculus | P26361 | - |
- |
Purification (Comment) | Organism |
---|---|
nucleotide-binding domain 1 and 2, purified to homogeneity under denaturing conditions | Homo sapiens |
nucleotide-binding domain 1 of CFTR purified to homogeneity by nickel ion affinity chromatography, followed by Sepharose S200 gel filtration and a second nickel affinity step | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
G551D mutant, with AMP | Mus musculus |
0.4 | - |
G551D mutant, with ATP | Mus musculus |
0.6 | - |
nucleotide-binding domain 2 of wild type, with ATP | Homo sapiens |
0.7 | - |
nucleotide-binding domain 1 of wild type, with AMP | Homo sapiens |
0.7 | - |
nucleotide-binding domain 1 of wild type, with ATP | Homo sapiens |
0.7 | - |
nucleotide-binding domain 2 of wild type, with ADP | Homo sapiens |
0.7 | - |
nucleotide-binding domain 2 of wild type, with AMP | Homo sapiens |
0.9 | - |
wild type, with AMP | Mus musculus |
1 | - |
G551D mutant, with ADP | Mus musculus |
1 | - |
nucleotide-binding domain 1 of wild type, with ADP | Homo sapiens |
1.2 | - |
wild type, with ATP | Mus musculus |
1.4 | - |
wild type, with ADP | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | GMP can not be substituted for the AMP substrate | Homo sapiens | ADP + phosphate | - |
r | |
AMP + H2O | GMP can not be substituted for the AMP substrate | Mus musculus | ADP + phosphate | - |
r | |
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
r | |
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
r | |
additional information | nucleotide-binding domain 1 cannot hydrolyze ATP | Mus musculus | ? | - |
? | |
additional information | nucleotide-binding domains 1 and 2 cannot hydrolyze ATP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | static light scattering | Mus musculus |
homodimer | Western blot analysis, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CFTR | - |
Homo sapiens |
CFTR | - |
Mus musculus |
cystic fibrosis transmembrane conductance regulator | - |
Homo sapiens |
cystic fibrosis transmembrane conductance regulator | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 3 h | Homo sapiens |
37 | - |
stable for 2.5 h | Mus musculus |