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Literature summary for 2.7.4.3 extracted from

  • Gross, C.H.; Abdul-Manan, N.; Fulghum, J.; Lippke, J.; Liu, X.; Prabhakar, P.; Brennan, D.; Willis, M.S.; Faerman, C.; Connelly, P.; Raybuck, S.; Moore, J.
    Nucleotide-binding domains of cystic fibrosis transmembrane conductance regulator, an ABC transporter, catalyze adenylate kinase activity but not ATP hydrolysis (2006), J. Biol. Chem., 281, 4058-4068.
    View publication on PubMed

Application

Application Comment Organism
additional information CFTR gating mechanism based on adenylate kinase activity Homo sapiens
additional information CFTR gating mechanism based on adenylate kinase activity Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
cloned into vector pET28b and expressed in Escherichia coli BL21DE3pLys cells Homo sapiens
cloned into vector pET28b and expressed in Escherichia coli BL21DE3pLys cells Mus musculus

Protein Variants

Protein Variants Comment Organism
G551D mutation affects the ability of the nucleotide-binding domain 1 of CFTR to dimerize, has an exaggerated nonlinear phase compared with the wild type Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
diadenosine polyphosphate inhibits adenylate kinase activity of the nucleotide-binding domains 1 and 2 of CFTR Homo sapiens
diadenosine polyphosphate inhibits adenylate kinase activity of the nucleotide-binding domain 1 of CFTR Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ATP wild type Mus musculus
0.03
-
AMP wild type Mus musculus
0.04
-
AMP nucleotide-binding domain 2 of wild type Homo sapiens
0.06
-
AMP nucleotide-binding domain 1 of wild type Homo sapiens
0.07
-
AMP G551D mutant Mus musculus
0.07
-
ATP nucleotide-binding domain 2 of wild type Homo sapiens
0.08
-
ATP G551D mutant Mus musculus
0.08
-
ATP nucleotide-binding domain 1 of wild type Homo sapiens
0.28
-
ADP nucleotide-binding domain 2 of wild type Homo sapiens
0.3
-
ADP nucleotide-binding domain 1 of wild type Homo sapiens
0.36
-
ADP G551D mutant Mus musculus
0.45
-
ADP wild type Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens P13569
-
-
Mus musculus P26361
-
-

Purification (Commentary)

Purification (Comment) Organism
nucleotide-binding domain 1 and 2, purified to homogeneity under denaturing conditions Homo sapiens
nucleotide-binding domain 1 of CFTR purified to homogeneity by nickel ion affinity chromatography, followed by Sepharose S200 gel filtration and a second nickel affinity step Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
G551D mutant, with AMP Mus musculus
0.4
-
G551D mutant, with ATP Mus musculus
0.6
-
nucleotide-binding domain 2 of wild type, with ATP Homo sapiens
0.7
-
nucleotide-binding domain 1 of wild type, with AMP Homo sapiens
0.7
-
nucleotide-binding domain 1 of wild type, with ATP Homo sapiens
0.7
-
nucleotide-binding domain 2 of wild type, with ADP Homo sapiens
0.7
-
nucleotide-binding domain 2 of wild type, with AMP Homo sapiens
0.9
-
wild type, with AMP Mus musculus
1
-
G551D mutant, with ADP Mus musculus
1
-
nucleotide-binding domain 1 of wild type, with ADP Homo sapiens
1.2
-
wild type, with ATP Mus musculus
1.4
-
wild type, with ADP Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O GMP can not be substituted for the AMP substrate Homo sapiens ADP + phosphate
-
r
AMP + H2O GMP can not be substituted for the AMP substrate Mus musculus ADP + phosphate
-
r
ATP + H2O
-
Homo sapiens ADP + phosphate
-
r
ATP + H2O
-
Mus musculus ADP + phosphate
-
r
additional information nucleotide-binding domain 1 cannot hydrolyze ATP Mus musculus ?
-
?
additional information nucleotide-binding domains 1 and 2 cannot hydrolyze ATP Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
homodimer static light scattering Mus musculus
homodimer Western blot analysis, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CFTR
-
Homo sapiens
CFTR
-
Mus musculus
cystic fibrosis transmembrane conductance regulator
-
Homo sapiens
cystic fibrosis transmembrane conductance regulator
-
Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable for 3 h Homo sapiens
37
-
stable for 2.5 h Mus musculus