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Literature summary for 2.7.4.3 extracted from
- Adenylate kinase from Thiobacillus neapolitanus. Unique properties, possibly designed to serve a unique metabolic function (1973), Biochim. Biophys. Acta, 321, 512-525.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol or various metal ions do not improve recovery during purification | Halothiobacillus neapolitanus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | substrate inhibition | Halothiobacillus neapolitanus |  |
| Mg2+ | at a high Mg:ATP ratio | Halothiobacillus neapolitanus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
ADP | cosubstrate ATP, pH 8.5 | Halothiobacillus neapolitanus | |
| 0.114 | 0.13 | AMP | pH 8.5 | Halothiobacillus neapolitanus | |
| 0.45 | 0.55 | ADP | pH 8.5 | Halothiobacillus neapolitanus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Halothiobacillus neapolitanus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Halothiobacillus neapolitanus | |
| Mg2+ | MgATP2- is true substrate | Halothiobacillus neapolitanus | |
| Mg2+ | MgADP- is true substrate | Halothiobacillus neapolitanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
gel filtration | Halothiobacillus neapolitanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halothiobacillus neapolitanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, to near homogeneity | Halothiobacillus neapolitanus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 79.5 | - |
pH 8.5 | Halothiobacillus neapolitanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Halothiobacillus neapolitanus | ATP + AMP | - |
r | |
| ATP + AMP | - |
Halothiobacillus neapolitanus | ADP + ADP | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
AMP + ATP | Halothiobacillus neapolitanus |
| 10 | - |
ADP + ADP | Halothiobacillus neapolitanus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | about half-maximal activity at pH 5.5 and about 60% of maximal activity at pH 10, AMP + ATP | Halothiobacillus neapolitanus |
| 8.5 | 11.5 | about half-maximal activity at pH 8.5 and 11.5, ADP + ADP | Halothiobacillus neapolitanus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
t1/2: 24 h, sodium phosphate buffer | Halothiobacillus neapolitanus |
| 6 | 9 | relatively stable, unstable below pH 5.5 | Halothiobacillus neapolitanus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Halothiobacillus neapolitanus | isoelectric focusing | - |
4.2 |
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Release 2023.1 (January 2023)
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