Any feedback?
Literature summary for 2.7.4.3 extracted from
- Zinc chelation and structural stability of adenylate kinase from Bacillus subtilis (1994), Biochemistry, 33, 9960-9967.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| adk-gene, expressed in Escherichia coli | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| enzyme from Bacillus stearothermophilus is more resistant to trypsin inactivation than that from E. coli or Bacillus subtilis | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-Hydroxymercuribenzene sulfonic acid | - |
Bacillus subtilis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 0.8-1 mol Zn2+ for wild-type and mutants H138N, D153C and D153T, 0.6 mol Zn2+ for mutant D153T, or 0.34 mol Zn2+ for mutant C130H per mol protein, atomic absorption spectrophotometry | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24100 | - |
calculated from nucleotide sequence | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed in E. coli | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 350 | 400 | - |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen enzyme solutions or lyophilized powders, wild-type and mutant enzymes, stable for weeks | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Bacillus subtilis | ATP + AMP | - |
r | |
| ADP + ADP | - |
Bacillus subtilis 168 | ATP + AMP | - |
r | |
| ATP + AMP | - |
Bacillus subtilis | ADP + ADP | - |
r | |
| ATP + AMP | - |
Bacillus subtilis 168 | ADP + ADP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000-27500, SDS-PAGE | Bacillus subtilis |
| monomer | 1 * 24100, calculated from nucleotide sequence | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
