Literature summary for 2.7.4.3 extracted from

Glaser, P.; Prescecan, E.; Delepierre, M.; Surewicz, W.K.; Mantsch, H.H.; B�rzu, O.; Gilles, A.M.
Zinc, a novel structural element found in the family of bacterial adenylate kinases (1992), Biochemistry, 31, 3038-3043.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned and expressed in Escherichia coli	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
5,5'-dithiobis(2-nitrobenzoic acid)	-	Escherichia coli
5,5'-dithiobis(2-nitrobenzoic acid)	-	Geobacillus stearothermophilus
AMP	-	Escherichia coli
AMP	-	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.036	0.037	ATP	ADP, 37°C	Geobacillus stearothermophilus
0.038	0.04	AMP	cosubstrate ATP, 30°C	Escherichia coli
0.048	0.051	ATP	cosubstrate AMP, 30°C	Escherichia coli
0.076	0.083	AMP	37°C	Geobacillus stearothermophilus
0.09	0.092	ADP3-	ADP, 30°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	not	Escherichia coli
Zn2+	requirement, tightly bound, 0.8 mol Zn2+ per mol protein, atomic absorption spectrophotometry	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24135	-	1 * 24135, calculated from nucleotide sequence	Geobacillus stearothermophilus
24140	-	calculated from nucleotide sequence	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Geobacillus stearothermophilus	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2400	-	-	Escherichia coli
3000	-	-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + ADP	-	Escherichia coli	ATP + AMP	-	r
ADP + ADP	-	Geobacillus stearothermophilus	ATP + AMP	-	r
ATP + AMP	-	Escherichia coli	ADP + ADP	-	r
ATP + AMP	-	Geobacillus stearothermophilus	ADP + ADP	-	r

Subunits

Subunits	Comment	Organism
monomer	-	Escherichia coli
monomer	1 * 24135, calculated from nucleotide sequence	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Escherichia coli
65	-	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)