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Literature summary for 2.7.4.3 extracted from
- Archaebacterial adenylate kinase from the thermoacidophile Sulfolobus acidocaldarius: purification, characterization, and partial sequence (1993), Arch. Biochem. Biophys., 302, 391-397.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| P1,P5-diadenosine 5'-pentaphosphate | weak | Sulfolobus acidocaldarius |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | 0.6 | AMP | cosubstrate ATP, 70°C | Sulfolobus acidocaldarius | |
| 0.65 | 0.7 | ADP | 70°C | Sulfolobus acidocaldarius | |
| 1.1 | - |
ATP | 90°C | Sulfolobus acidocaldarius | |
| 1.3 | 1.37 | AMP | 90°C | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ | Sulfolobus acidocaldarius | |
| Ca2+ | in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ | Sulfolobus acidocaldarius | |
| Ca2+ | residual activity even in the presence of EDTA | Sulfolobus acidocaldarius | |
| Mg2+ | requirement | Sulfolobus acidocaldarius | |
| Mg2+ | in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ | Sulfolobus acidocaldarius | |
| Mg2+ | in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ | Sulfolobus acidocaldarius | |
| Mg2+ | residual activity even in the presence of EDTA | Sulfolobus acidocaldarius | |
| Mn2+ | in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ | Sulfolobus acidocaldarius | |
| Mn2+ | in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ | Sulfolobus acidocaldarius | |
| Mn2+ | residual activity even in the presence of EDTA | Sulfolobus acidocaldarius | |
| Zn2+ | in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ | Sulfolobus acidocaldarius | |
| Zn2+ | in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ | Sulfolobus acidocaldarius | |
| Zn2+ | residual activity even in the presence of EDTA | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
2 * 23500, SDS-PAGE | Sulfolobus acidocaldarius |
| 52000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.15 | - |
45°C | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Sulfolobus acidocaldarius | ATP + AMP | - |
r | |
| ATP + AMP | no substrates are GTP/GMP, TTP/TMP | Sulfolobus acidocaldarius | ADP + ADP | - |
r | |
| ATP + AMP | highly specific for AMP | Sulfolobus acidocaldarius | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/UMP | Sulfolobus acidocaldarius | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/TMP | Sulfolobus acidocaldarius | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/GMP | Sulfolobus acidocaldarius | ADP + ADP | - |
r | |
| CTP + AMP | reaction at 68% the rate of ATP | Sulfolobus acidocaldarius | ADP + CDP | - |
? | |
| dATP + AMP | reaction at 80% the rate of ATP | Sulfolobus acidocaldarius | dADP + ADP | - |
? | |
| GTP + AMP | reaction at 71% the rate of AMP | Sulfolobus acidocaldarius | ADP + GDP | - |
? | |
| ITP + AMP | reaction at 58% the rate of ATP | Sulfolobus acidocaldarius | IDP + ADP | - |
? | |
| UTP + AMP | reaction at 53% the rate of AMP | Sulfolobus acidocaldarius | ADP + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23500, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | about half-maximal activity at 70°C and about 75% of maximal activity at 95°C | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
24 h, stable at pH 6 | Sulfolobus acidocaldarius |
| 65 | 70 | 12 h, 16% loss of activity, 24 h, 23% loss of activity, variation of ionic strength or addition of substrates does not stabilize | Sulfolobus acidocaldarius |
| 85 | - |
3 h, inactivation | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
AMP + ATP, 70°C | Sulfolobus acidocaldarius |
| 6 | - |
AMP + ATP, 70°C | Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | about half-maximal activity at pH 3.5 and about 70% of maximal activity at pH 7, ADP + ADP, 70°C | Sulfolobus acidocaldarius |
| 4 | 7.5 | about half-maximal activity at pH 4 and about 70% of maximal activity at pH 7.5, AMP + ATP, 70°C | Sulfolobus acidocaldarius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 3 | 2 h, up to 15% loss of activity | Sulfolobus acidocaldarius |
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