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Literature summary for 2.7.4.3 extracted from

  • Tsuboi, K.K.; Chervenka, C.H.
    Adenylate kinase of human erythrocyte. Isolation and properties of the predominant inherited form (1975), J. Biol. Chem., 250, 132-140.
    View publication on PubMed
Search for more literature for 2.7.4.3

General Stability

General Stability Organism
low ionic strength inactivates Homo sapiens
Triton X-100, EDTA, dithiothreitol and electrolyte protect enzyme in dilute solution and against denaturation by heat or extreme pH-values Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP product inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076 0.083 AMP 25°C, pH 8.0 Homo sapiens
0.09 0.092 ADP3- 25°C, pH 8.0 Homo sapiens
0.09 0.092 ADP3- ATP production Homo sapiens
0.11
-
 ADP 25°C, pH 8.0 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens
Mg2+ 1 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21300
-
 sedimentation equilibrium Homo sapiens
21300
-
 isozyme AKalpha, sedimentation equilibrium Homo sapiens
22500
-
 isozyme AKalpha, gel filtration Homo sapiens
23000
-
 1 * 23000, isozyme AKalpha, SDS-PAGE Homo sapiens
23400
-
 1 * 23400, isozyme AKalpha, sedimentation equilibrium in 6 M guanidine hydrochloride Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
predominant form AKalpha (major form of AK-1 isozymes) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3200
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
0°C, a few days stable, after which activity drops to 70%, this lower specific activity can be maintained at -20°C in ammonium sulfate solution for a prolonged period Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + ADP
-
 Homo sapiens ATP + AMP
-
 r
ATP + AMP best substrates Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates are ATP/dGMP Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates are ATP/IMP Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates are dGTP/AMP Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates are ATP/UMP Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates are ATP/GMP Homo sapiens ADP + ADP
-
 r
ATP + CMP reaction at 1% the rate of AMP Homo sapiens ADP + ?
-
 ?
ATP + dAMP reaction at 10% the rate of AMP Homo sapiens ADP + dADP
-
 ?
CTP + AMP reaction at about 3% the rate of ATP Homo sapiens ADP + CDP
-
 ?
dATP + AMP reaction at about 50% the rate of ATP Homo sapiens dADP + ADP
-
 ?
ITP + AMP not ATP/IMP Homo sapiens IDP + ADP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23400, isozyme AKalpha, sedimentation equilibrium in 6 M guanidine hydrochloride Homo sapiens
monomer 1 * 23000, isozyme AKalpha, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 in 10 mM phosphate buffer, pH 7, 0.1 M KCl, 0.02% Triton X-100, 2 mM DTT, +/- EDTA, t1/2: 10 min, with more than 0.2 M KCl: 10-20% loss of activity within 10 min Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 10 min, at 90°C, in 10 mM phosphate buffer, pH 7, 0.1 M KCl, DTT, Triton X-100, +/- EDTA, 10% loss of activity within 10 min Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isozyme AKalpha, isoelectric focusing
-
 9