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Literature summary for 2.7.4.3 extracted from
- Preparation and characterization of a crystalline human ATP:AMP phosphotransferase (1972), Biochim. Biophys. Acta, 268, 81-91.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| muscle | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | 0.3 | ATP | 25°C, pH 8.0 | Homo sapiens |  |
| 0.32 | - |
AMP | 25°C, pH 8.0 | Homo sapiens | |
| 0.34 | 0.35 | ADP | 25°C, pH 8.0 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Homo sapiens | |
| Mg2+ | maximal activity when MgCl2/ADP-ratio: about 0.5 and MgCl2/ATP-ratio: 1 | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21500 | - |
muscle, gel filtration | Homo sapiens |
| 21700 | - |
amino acid analysis | Homo sapiens |
| 22600 | - |
titration of 2 SH-groups | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1920 | - |
25°C, pH 8.0 | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in distilled water, 6 weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Homo sapiens | ATP + AMP | - |
r | |
| ATP + AMP | specificity for AMP-site is much more rigorous than for ATP-site | Homo sapiens | ADP + ADP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21500, SDS-PAGE | Homo sapiens |
| monomer | 1 * 21700, calculated from amino acid analysis | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 683 | - |
ATP | - |
Homo sapiens | |
| 783 | - |
ADP | - |
Homo sapiens | |
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