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Literature summary for 2.7.4.26 extracted from

  • Dellas, N.; Noel, J.P.
    Mutation of archaeal isopentenyl phosphate kinase highlights mechanism and guides phosphorylation of additional isoprenoid monophosphates (2010), Chem. Biol., 5, 589-601.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray structure determination Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
F83A/I86A/I156A the mutant shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. The reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii
H60Q His60 plays a key role in binding and catalysis, the mutant enzyme shows increased values for Km and decreased vaules for kcat and kcat/Km Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
0.0345
-
isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
0.198
-
ATP wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
0.559
-
ATP mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + isopentenyl phosphate Methanocaldococcus jannaschii
-
ADP + isopentenyl diphosphate
-
?
ATP + isopentenyl phosphate Methanocaldococcus jannaschii DSM 2661
-
ADP + isopentenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q60352
-
-
Methanocaldococcus jannaschii DSM 2661 Q60352
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (2E,6E)-farnesyl phosphate the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii ADP + (2E,6E)-farnesyl diphosphate
-
?
ATP + (2E,6E)-farnesyl phosphate the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii DSM 2661 ADP + (2E,6E)-farnesyl diphosphate
-
?
ATP + isopentenyl phosphate
-
Methanocaldococcus jannaschii ADP + isopentenyl diphosphate
-
?
ATP + isopentenyl phosphate
-
Methanocaldococcus jannaschii DSM 2661 ADP + isopentenyl diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
1.46
-
isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
340
-
isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii