Crystallization (Comment) | Organism |
---|---|
X-ray structure determination | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
F83A/I86A/I156A | the mutant shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. The reaction is not catalyzed by the wild-type enzyme | Methanocaldococcus jannaschii |
H60Q | His60 plays a key role in binding and catalysis, the mutant enzyme shows increased values for Km and decreased vaules for kcat and kcat/Km | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
isopentenyl phosphate | wild-type enzyme, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.0345 | - |
isopentenyl phosphate | mutant H60Q, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.198 | - |
ATP | wild-type enzyme, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.559 | - |
ATP | mutant H60Q, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + isopentenyl phosphate | Methanocaldococcus jannaschii | - |
ADP + isopentenyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | Methanocaldococcus jannaschii DSM 2661 | - |
ADP + isopentenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q60352 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q60352 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2E,6E)-farnesyl phosphate | the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme | Methanocaldococcus jannaschii | ADP + (2E,6E)-farnesyl diphosphate | - |
? | |
ATP + (2E,6E)-farnesyl phosphate | the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme | Methanocaldococcus jannaschii DSM 2661 | ADP + (2E,6E)-farnesyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | - |
Methanocaldococcus jannaschii | ADP + isopentenyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | - |
Methanocaldococcus jannaschii DSM 2661 | ADP + isopentenyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
isopentenyl phosphate | mutant H60Q, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
1.46 | - |
isopentenyl phosphate | wild-type enzyme, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
isopentenyl phosphate | mutant H60Q, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
340 | - |
isopentenyl phosphate | wild-type enzyme, pH 8.0, temperature not specified in the publication | Methanocaldococcus jannaschii |