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Literature summary for 2.7.4.25 extracted from

  • Thum, C.; Schneider, C.Z.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP (2009), J. Bacteriol., 191, 2884-2887.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis
0.165
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
13.85
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 gel filtration Mycobacterium tuberculosis
23930
-
 ESI-MS Mycobacterium tuberculosis
24060
-
 calculated from amino acid sequence Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-80°C, no apparent loss of activity Mycobacterium tuberculosis
4°C, up to 30 days, recombinant CMK remains stable Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis ADP + CDP
-
 ?
ATP + CMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis H37Rv ADP + CDP
-
 ?
ATP + dCMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis ADP + dCDP
-
 ?
ATP + dCMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis H37Rv ADP + dCDP
-
 ?
ATP + UMP UMP is a poor substrate Mycobacterium tuberculosis ADP + UDP
-
 ?
ATP + UMP UMP is a poor substrate Mycobacterium tuberculosis H37Rv ADP + UDP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 21000, gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CMK
-
 Mycobacterium tuberculosis
CMP kinase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.2
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis
30
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
52
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.88
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis
200
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
1100
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis