Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K248A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 | Homo sapiens |
R213A | the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000022 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.00006 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.00011 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
0.0052 | - |
ADP | isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.022 | - |
ATP | isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.9 | - |
ADP | isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43314 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap HP column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | - |
Homo sapiens | ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
r | |
ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | - |
Homo sapiens | ATP + 1D-myo-inositol hexakisphosphate | - |
r | |
ATP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
Homo sapiens | ADP + 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
diphosphoinositol pentakisphosphate kinase 2 | - |
Homo sapiens |
PPIP5K2 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
0.13 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.19 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
ADP | isoform PPIP5K2, using 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
0.21 | - |
ADP | isoform PPIP5K2, using 1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
5.9 | - |
ATP | isoform PPIP5K2, using 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
17 | - |
1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C | Homo sapiens | |
2200 | - |
5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens | |
8500 | - |
1,5-bis-diphospho-1D-myo-inositol 2,3,4,6-tetrakisphosphate | isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C | Homo sapiens |