Literature summary for 2.7.4.21 extracted from

Nagata, E.; Nonaka, T.; Moriya, Y.; Fujii, N.; Okada, Y.; Tsukamoto, H.; Itoh, J.; Okada, C.; Satoh, T.; Arai, T.; Hasegawa, M.; Takizawa, S.
Inositol hexakisphosphate kinase 2 promotes cell death in cells with cytoplasmic TDP-43 aggregation (2016), Mol. Neurobiol., 53, 5377-5383.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant co-overexpression of InsP6K2 and C-terminal residues 219-414 of TDP-43 from InsP6K2-pDsRed tag and TDP219-pEGFP tag plasmids in SHSY-5Y cells. Transfection of SHSY-5Y cells with a plasmid encoding a catalytically inactive, dominant-negative InsP6K2 (K2 K/A). HSP90 protein expression is reduced in the cytoplasmic fraction of TDP219- and InsP6K2-transfected cells. HSP90 expression is also significantly reduced in cells doubly transfected with TDP219M and InsP6K2 compared with control cells	Homo sapiens

Cloned (Comment)

Organism

recombinant co-overexpression of InsP6K2 and C-terminal residues 219-414 of TDP-43 from InsP6K2-pDsRed tag and TDP219-pEGFP tag plasmids in SHSY-5Y cells. Transfection of SHSY-5Y cells with a plasmid encoding a catalytically inactive, dominant-negative InsP6K2 (K2 K/A). HSP90 protein expression is reduced in the cytoplasmic fraction of TDP219- and InsP6K2-transfected cells. HSP90 expression is also significantly reduced in cells doubly transfected with TDP219M and InsP6K2 compared with control cells

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	enzyme InsP6K2 is translocated from the nucleus to the cytosol during apoptosis	Homo sapiens	5829	-
nucleus	enzyme InsP6K2 is translocated from the nucleus to the cytosol during apoptosis	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UHH9	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
anterior horn cell	spinal cord	Homo sapiens	-
spinal cord	co-localization with phosphorylated TDP-43 of InsP6K2 in the cytoplasm of anterior horn cells of the spinal cord	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
inositol hexakisphosphate kinase type 2	-	Homo sapiens
InsP6K2	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	enzyme InsP6K2 is translocated from the nucleus to the cytosol during apoptosis	Homo sapiens
physiological function	inositol hexakisphosphate kinase type 2 (InsP6K2), which converts inositol hexakisphosphate (InsP6) to InsP7, mediates cell death in mammalian cells. Cell death is augmented in the presence of cytoplasmic TDP-43 aggregations and activated InsP6K2, while cells with only cytoplasmic TDP-43 aggregation survive because Akt activity increases. Enzyme InsP6K2 causes neuronal cell death in patients suffering frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U) or amyotrophic lateral sclerosis (ALS). InsP6K2 and cytoplasmic TDP-43 induce depletion of Akt phosphorylation and decrease casein kinase 2	Homo sapiens