Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.21 extracted from

  • Yu, W.; Ye, C.; Greenberg, M.L.
    Inositol hexakisphosphate kinase 1 (IP6K1) regulates inositol synthesis in mammalian cells (2016), J. Biol. Chem., 291, 10437-10444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.21

Cloned(Commentary)

Cloned (Comment) Organism
gene Ip6k1, expression of murine IP6K1 rescues yeast kcs1DELTA inositol auxotrophy. Nuclear recombinant expression of GFP-tagged wild type IP6K1 in IP6K1 knockout cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of IP6K1 knockout MEF cells. Deletion of the Q2 domain results in decreased nuclear localization in more than 60% of cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mus musculus 5737
-
additional information IP6K1 exhibits dual localization in the cytoplasm and nucleus Mus musculus
-
-
nucleus enzyme IP6K1 requires phosphatidic acid binding for nuclear localization and repression of mIno1 transcription. Deletion of the Q2 domain results in decreased nuclear localization in more than 60% of cells Mus musculus 5634
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q6PD10
-
-

Source Tissue

Source Tissue Comment Organism Textmining
MEF cell
-
 Mus musculus
-

Synonyms

Synonyms Comment Organism
inositol hexakisphosphate kinase 1
-
 Mus musculus
IP6K1
-
 Mus musculus

General Information

General Information Comment Organism
malfunction iInositol synthesis is up-regulated in IP6K1 knockout MEF cells. Ip6k1 ablation leads to profound changes in DNA methylation and expression of Isyna1 (designated mIno1), which encodes the rate-limiting enzyme inositol-3-phosphate synthase. Expression of Q3DELTA, which lacks both the HOPA domain and the catalytic motif, leads to increased mIno1 mRNA levels in IP6K1-KO cells. In the mIno1 promoter region, most of the CpG sites exhibit a similar, but slightly decreased, pattern of methylation in IP6K1 knockout cells compared with wild-type cells. The CpG sites between the first and second ATGs exhibit markedly less methylation in IP6K1 knockout cells. Deletion of the Q4 domain of the enzyme results in increased mIno1 expression Mus musculus
additional information the catalytic motif of enzyme IP6K1 lies in the Q3 domain Mus musculus
physiological function IP6K1, an inositol hexakisphosphate kinase that catalyzes the synthesis of inositol diphosphate, regulates inositol synthesis in mammalian cells. Enzyme IP6K1 may negatively regulate mIno1 transcription by increasing the methylation of mIno1 DNA. The catalytic function of IP6K1 is necessary for repression of mIno1 transcription Mus musculus