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Literature summary for 2.7.4.2 extracted from
- Pig liver phosphomevalonate kinase: kinetic mechanism (2006), Arch. Biochem. Biophys., 454, 188-195.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | detailed kinetics, Bi Bi sequential ordered mechanism with phosphomevalonate as the first substrate and ADP the last product, the enzyme follows hyperbolic kinetics and a sequential mechanism, initial velocity and isotope exchange at equilibrium measurements, overview | Sus scrofa | |
| 0.012 | - |
(R)-5-phosphomevalonate | pH 7.5, 30°C | Sus scrofa |  |
| 0.043 | - |
ATP | pH 7.5, 30°C | Sus scrofa | |
| 0.043 | - |
ATP | ATP in form of MgATP2- | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme requires cations with Mg2+ being the most effective | Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-5-phosphomevalonate | Sus scrofa | one of the initial steps in the biosynthesis of steroids and isoprenoids | ADP + (R)-5-diphosphomevalonate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from liver | Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate | kinetic mechanism, the enzyme follows hyperbolic kinetics and a sequential mechanism | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
purified enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-5-phosphomevalonate | - |
Sus scrofa | ADP + (R)-5-diphosphomevalonate | - |
r | |
| ATP + (R)-5-phosphomevalonate | one of the initial steps in the biosynthesis of steroids and isoprenoids | Sus scrofa | ADP + (R)-5-diphosphomevalonate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000-22500, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphomevalonate kinase | - |
Sus scrofa |
| PMK | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Sus scrofa | |
| ATP | - |
Sus scrofa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, product inhibition patterns | Sus scrofa |
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