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Literature summary for 2.7.4.2 extracted from

  • Bazaes, S.; Beytia, E.; Jabalquinto, A.M.; Solis de Ovando, F.; Gomez, I.; Eyzaguirre, J.
    Pig liver phosphomevalone kinase. 1. Purification and properties (1980), Biochemistry, 19, 2300-2304.
    View publication on PubMed
Search for more literature for 2.7.4.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 phosphomevalonate
-
 Sus scrofa
0.46
-
 ATP
-
 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Sus scrofa
Co2+ 63.6% of the activity with Mg2+ Sus scrofa
Mg2+ maximal activity at 5 mM Sus scrofa
Mg2+ divalent cation required, Mg2+ most efficient Sus scrofa
Mn2+ can partially replace Mg2+ in activation Sus scrofa
Mn2+ 62.2% of the activity with Mg2+ Sus scrofa
Zn2+ can partially replace Mg2+ in activation Sus scrofa
Zn2+ 65.1% of the activity with Mg2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 1 * 21000, SDS-PAGE Sus scrofa
22000
-
 sucrose density gradient centrifugation Sus scrofa
22500
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Sus scrofa one of the enzymes in the biosynthetic pathway of the polyisoprenoid precursor isopentenyl diphosphate, enzyme has no regulatory function in cholesterol biosynthesis in liver ADP + 5-diphosphomevalonate
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate sequential mechanism Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.1
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris or phosphate buffer, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, 60 days, no loss of activity Sus scrofa
-20°C, 50% glycerol Sus scrofa
4°C, 10 mM 2-mercaptoethanol, 3 months, no loss of activity Sus scrofa
4°C, 20 days, 99% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate one of the enzymes in the biosynthetic pathway of the polyisoprenoid precursor isopentenyl diphosphate, enzyme has no regulatory function in cholesterol biosynthesis in liver Sus scrofa ADP + 5-diphosphomevalonate
-
 r
ATP + 5-phosphomevalonate absolute specificity for ATP Sus scrofa ADP + 5-diphosphomevalonate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 21000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 approx. 30% of maximal activity at pH 6.0 Sus scrofa