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Literature summary for 2.7.4.16 extracted from

  • Kim, H.J.; Lee, H.; Lee, Y.; Choi, I.; Ko, Y.; Lee, S.; Jang, S.
    The ThiL enzyme is a valid antibacterial target essential for both thiamine biosynthesis and salvage pathways in Pseudomonas aeruginosa (2020), J. Biol. Chem., 295, 10081-10091 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxyindolacetic acid uncompetitive inhibitor Pseudomonas aeruginosa
WAY213613 noncompetitive inhibitor Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 thiamine phosphate pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.111
-
 ATP pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.152
-
 oxythiamine monophosphate pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thiamine phosphate Pseudomonas aeruginosa
-
 ADP + thiamine diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxythiamine monophosphate
-
 Pseudomonas aeruginosa ADP + ?
-
 ?
ATP + thiamine phosphate
-
 Pseudomonas aeruginosa ADP + thiamine diphosphate
-
 ?
ATP + thiamine phosphate random bi-bi mechanism. Thiamine diphosphate is not further phosphorylated by ThiL Pseudomonas aeruginosa ADP + thiamine diphosphate
-
 ?
additional information oxythiamine, pyrithiamine, and amprolium, are not phosphorylated Pseudomonas aeruginosa ?
-
-

Synonyms

Synonyms Comment Organism
thiamine monophosphate kinase
-
 Pseudomonas aeruginosa
ThiL
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0134
-
 WAY213613 pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.114
-
 5-hydroxyindolacetic acid pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa

General Information

General Information Comment Organism
drug target the enzyme represents a potential drug target for the development of an augmented repertoire of antibiotics against Pseudomonas aeruginosa Pseudomonas aeruginosa