General Stability | Organism |
---|---|
glycerol stabilizes during purification | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | product inhibition | Escherichia coli | |
AMP | - |
Escherichia coli | |
Cs+ | antagonizes stimulation by K+ | Escherichia coli | |
diphosphate | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
Li+ | antagonizes stimulation by K+ | Escherichia coli | |
additional information | no inhibition by phosphate, KCN, iodoacetic acid, arsenate or arsenite | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
Na+ | antagonizes stimulation by K+ | Escherichia coli | |
NaF | weak | Escherichia coli | |
oxythiamine | - |
Escherichia coli | |
PCMB | 2-mercaptoethanol reverses | Escherichia coli | |
pyrithiamine | - |
Escherichia coli | |
Pyrithiamine phosphate | - |
Escherichia coli | |
thiamine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
thiamine phosphate | pH 7.5, 37°C | Escherichia coli | |
0.27 | - |
ATP | pH 7.5, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement, about 95% as effective as Mg2+ | Escherichia coli | |
Fe2+ | activation, about 10% as effective as Mg2+ | Escherichia coli | |
K+ | activation | Escherichia coli | |
Mg2+ | requirement | Escherichia coli | |
Mn2+ | activation, about 30% as effective as Mg2+ | Escherichia coli | |
additional information | no activation by Ba2+, Ca2+, Cu2+, Cs+, Li+, Na+ | Escherichia coli | |
NH4+ | activation | Escherichia coli | |
Rb+ | activation | Escherichia coli | |
Zn2+ | activation, about 30% as effective as Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine phosphate | Escherichia coli | - |
ADP + thiamine diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000197 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 3 days, t1/2: 10 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + thiamine phosphate | poor substrate | Escherichia coli | ? | - |
? | |
ATP + thiamine phosphate | - |
Escherichia coli | ADP + thiamine diphosphate | - |
? | |
ATP + thiamine phosphate | no substrates: thiamine, GTP, CTP, UTP, AMP | Escherichia coli | ADP + thiamine diphosphate | - |
? | |
ITP + thiamine phosphate | poor substrate | Escherichia coli | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
pyrithiamine | pH 7.5, 37°C | Escherichia coli |