Cloned (Comment) | Organism |
---|---|
UMP-CMPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged mutant UMP-CMPK2DELTA21, lacking the mitochondrial targeting sequence, in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, expression of GFP-tagged wild-type and E448G mutant enzymes in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E448G | site-directed mutagenesis | Homo sapiens |
additional information | construction of a UMP-CMPK2DELTA21 mutant lacking the mitochondrial targeting sequence | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
dUMP | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
1.31 | - |
dCMP | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
3.09 | - |
CMP | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
6.3 | - |
UMP | pH 8.0, 37°C, wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | UMP-CMPK2 contains an N-terminal mitochondrial targeting sequence | Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q5EBM0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged mutant UMP-CMPK2DELTA21 from Spodoptera frugiperda Sf9 cells by affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
K-562 cell | chronic myelogenous leukemia cell line | Homo sapiens | - |
leukemia cell | high expression | Homo sapiens | - |
macrophage | - |
Homo sapiens | - |
MOLT-4 cell | lymphoblastic leukemia cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | low activity | Homo sapiens | ADP + CDP | - |
? | |
ATP + dCMP | high activity | Homo sapiens | ADP + dCDP | - |
? | |
ATP + dUMP | best substrate | Homo sapiens | ADP + dUDP | - |
? | |
ATP + UMP | low activity | Homo sapiens | ADP + UDP | - |
? | |
additional information | UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | mitochondrial UMP-CMPK2 belongs to a distinct nucleoside monophosphate kinase family, closer to thymidylate kinase than to cytosolic UMP-CMP kinase | Homo sapiens |
nucleoside monophosphate kinase | - |
Homo sapiens |
UMP-CMP kinase 2 | - |
Homo sapiens |
UMP-CMPK2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |