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show all sequences of 2.7.4.13

Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13)

Mikoulinskaia, G.V.; Zimin, A.A.; Feofanov, S.A.; Miroshnikov, A.I.; Protein Expr. Purif. 33, 166-175 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Escherichia virus T5
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28670
-
calculated from nucleotid sequence
Escherichia virus T5
29140
-
SDS-PAGE
Escherichia virus T5
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside monophosphate
Escherichia virus T5
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia virus T5
Q6QGP4
-
-
Purification (Commentary)
Commentary
Organism
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases
Escherichia virus T5
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29
-
recombinant protein in sonicated cell exctracts, with dGMP as substrate
Escherichia virus T5
36
-
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate
Escherichia virus T5
40
-
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate
Escherichia virus T5
84.2
-
homogeneous recombinant enzyme with dCMP as substrate
Escherichia virus T5
153.6
-
homogeneous recombinant enzyme with dGMP as substrate
Escherichia virus T5
155
-
affinity chromatography eluant of the recombinant protein, with dGMP as substrate; ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate
Escherichia virus T5
166.8
-
homogeneous recombinant enzyme with dTMP as substrate
Escherichia virus T5
232
-
homogeneous recombinant enzyme with dAMP as substrate
Escherichia virus T5
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
-
663294
Escherichia virus T5
ADP + dADP
-
-
-
?
ATP + dCMP
-
663294
Escherichia virus T5
ADP + dCDP
-
-
-
?
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
663294
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
-
-
?
ATP + dGMP
-
663294
Escherichia virus T5
ADP + dGDP
-
-
-
?
ATP + dTMP
-
663294
Escherichia virus T5
ADP + dTDP
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Escherichia virus T5
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28670
-
calculated from nucleotid sequence
Escherichia virus T5
29140
-
SDS-PAGE
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside monophosphate
Escherichia virus T5
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29
-
recombinant protein in sonicated cell exctracts, with dGMP as substrate
Escherichia virus T5
36
-
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate
Escherichia virus T5
40
-
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate
Escherichia virus T5
84.2
-
homogeneous recombinant enzyme with dCMP as substrate
Escherichia virus T5
153.6
-
homogeneous recombinant enzyme with dGMP as substrate
Escherichia virus T5
155
-
affinity chromatography eluant of the recombinant protein, with dGMP as substrate; ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate
Escherichia virus T5
166.8
-
homogeneous recombinant enzyme with dTMP as substrate
Escherichia virus T5
232
-
homogeneous recombinant enzyme with dAMP as substrate
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
-
663294
Escherichia virus T5
ADP + dADP
-
-
-
?
ATP + dCMP
-
663294
Escherichia virus T5
ADP + dCDP
-
-
-
?
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
663294
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
-
-
?
ATP + dGMP
-
663294
Escherichia virus T5
ADP + dGDP
-
-
-
?
ATP + dTMP
-
663294
Escherichia virus T5
ADP + dTDP
-
-
-
?
Other publictions for EC 2.7.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739645
Mikoulinskaia
-
The study of the bacteriophage ...
Escherichia virus T5
Russ. J. Bioorg. Chem.
39
607-618
2013
-
-
1
-
15
-
-
-
-
1
-
4
-
1
-
-
1
-
-
-
5
-
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
15
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
5
-
9
1
1
-
-
-
2
-
-
-
-
1
1
-
-
-
661371
Bao
Cloning of deoxynucleoside mon ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
93
572-580
2006
-
-
1
-
-
-
-
8
-
1
4
1
-
3
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
1
4
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
663294
Mikoulinskaia
Identification, cloning, and e ...
Escherichia virus T5
Protein Expr. Purif.
33
166-175
2004
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642544
Mikoulinskaia
Purification and characterizat ...
Escherichia virus T5, no activity in Escherichia coli
Protein Expr. Purif.
27
195-201
2003
-
-
-
-
-
-
-
-
-
-
2
1
-
4
-
-
1
-
-
-
2
1
5
1
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
2
1
5
1
-
-
4
-
1
1
-
1
-
-
-
-
-
-
209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4, no activity in Escherichia coli
J. Biol. Chem.
258
5746-5753
1983
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642542
Bessman
The enzymology of virus-infect ...
Escherichia virus T5, no activity in Escherichia coli
J. Biol. Chem.
240
439-445
1965
-
-
-
-
-
-
3
6
-
5
-
1
-
2
-
-
1
-
-
-
1
2
12
-
1
-
-
-
2
3
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
6
-
5
-
1
-
-
-
1
-
-
1
2
12
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-