Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, SDS-PAGE | Tequintavirus T5 |
29140 | - |
analytical equilibrium ultracentrifugation | Tequintavirus T5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxynucleoside phosphate | Tequintavirus T5 | enzyme induced during viral infection | ADP + deoxynucleoside diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Escherichia coli | - |
- |
- |
Tequintavirus T5 | - |
from infected Escherichia coli cells | - |
Tequintavirus T5 | - |
mutant T5amH128a | - |
Purification (Comment) | Organism |
---|---|
1190fold to homogeneity | Tequintavirus T5 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
166.8 | - |
purified enzyme, substrate dTMP | Tequintavirus T5 |
232 | - |
purified enzyme, substrate dAMP | Tequintavirus T5 |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months | Tequintavirus T5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dAMP | dAMP is the best acceptor substrate | Tequintavirus T5 | ADP + dADP | - |
r | |
ATP + dCMP | phosphorylated at 36% the rate of dAMP | Tequintavirus T5 | ADP + dCDP | - |
r | |
ATP + deoxynucleoside phosphate | enzyme induced during viral infection | Tequintavirus T5 | ADP + deoxynucleoside diphosphate | - |
r | |
ATP + dGMP | phosphorylated at 65% the rate of dAMP | Tequintavirus T5 | ADP + dGDP | - |
r | |
ATP + dTMP | phosphorylated at 70% the rate of dAMP | Tequintavirus T5 | ADP + dTDP | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Tequintavirus T5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
60 min, stable | Tequintavirus T5 |
42 | - |
10% loss of activity after 30 min | Tequintavirus T5 |
47 | - |
50% loss of activity after 30 min | Tequintavirus T5 |
52 | - |
rapid inactivation, t1/2: 5 min | Tequintavirus T5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Tequintavirus T5 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active over a broad range | Tequintavirus T5 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tequintavirus T5 | determined under partially denaturing condiions | - |
4.2 |