BRENDA - Enzyme Database
show all sequences of 2.7.4.10

Purification and properties of nucleoside triphosphate-adenosine monophosphate transphosphorylase from beef heart mitochondria

Albrecht, G.J.; Biochemistry 9, 2462-2470 (1970)

Data extracted from this reference:

General Stability
General Stability
Organism
ammonium sulfate or substrates enhance stability
Bos taurus
freeze-thawing, dilutions, or low ionic strength decreases activity rapidly
Bos taurus
stability increases during purification
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
reverse reaction, kinetics
Bos taurus
AMP
free form, Mg2+ reverses
Bos taurus
ATP
-
Bos taurus
GDP
reverse reaction, kinetics
Bos taurus
GTP
kinetics
Bos taurus
Mg2+
weak, above 4 mM, activates below
Bos taurus
additional information
no inhibition by EDTA, GSH, cysteamine or high phosphate concentrations
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
GDP
pH 8.0, with ADP
Bos taurus
0.033
-
AMP
-
Bos taurus
0.056
-
GTP
pH 8.0, with AMP
Bos taurus
0.29
-
ADP
pH 8.0, with GDP
Bos taurus
0.63
-
ITP
pH 8.0, with AMP
Bos taurus
1
-
ATP
pH 8.0, with of AMP
Bos taurus
7.4
-
UTP
pH 8.0, with of AMP
Bos taurus
9.1
-
CTP
pH 8.0, with of AMP
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
matrix
Bos taurus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, 50% as effective as Mg2+
Bos taurus
Mg2+
activation, 4 mM, as good as Mn2+, slightly inibitory above 4 mM
Bos taurus
Mn2+
activation, as good as Mg2+
Bos taurus
additional information
no activation by EDTA, cysteamine, GSH or high phosphate concentrations
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Reaction
Reaction
Commentary
Organism
nucleoside triphosphate + AMP = nucleoside diphosphate + ADP
mechanism
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
135
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
0°C, partially purified preparation, several weeks
Bos taurus
room temperature, 1 week
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + AMP
poor substrate, highly specific for AMP, phosphorylation at about 5% the rate of ITP
642534
Bos taurus
ADP
-
-
-
r
CTP + AMP
highly specific for AMP, phosphorylation at about 11% the rate of ITP
642534
Bos taurus
CDP + ADP
-
-
-
r
GTP + AMP
highly specific for AMP and ADP, in reverse reaction
642534
Bos taurus
GDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
GTP + AMP
phosphorylation at about 90% the rate of ITP
642534
Bos taurus
GDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
best substrate
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
highly specific for AMP and ADP in reverse reaction
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
no acceptor substrates are 3'-GMP
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
not: IMP, CMP, UMP
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
UTP + AMP
highly specific for AMP, phosphorylation at about 19% the rate of ITP
642534
Bos taurus
UDP + ADP
-
-
-
r
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
35% ammonium sulfate, AMP or other substrates enhance thermal stability
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.7
about half-maximal activity at pH 7 and 9.7
Bos taurus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.73
-
ATP
pH 8.5, competitive with AMP
Bos taurus
0.74
-
ATP
pH 8.5, competitive with GTP
Bos taurus
0.77
-
GDP
pH 8.5
Bos taurus
0.8
-
GTP
pH 8.5
Bos taurus
0.9
-
ADP
pH 8.5
Bos taurus
1.6
-
AMP
pH 8.5
Bos taurus
General Stability (protein specific)
General Stability
Organism
ammonium sulfate or substrates enhance stability
Bos taurus
freeze-thawing, dilutions, or low ionic strength decreases activity rapidly
Bos taurus
stability increases during purification
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
reverse reaction, kinetics
Bos taurus
AMP
free form, Mg2+ reverses
Bos taurus
ATP
-
Bos taurus
GDP
reverse reaction, kinetics
Bos taurus
GTP
kinetics
Bos taurus
Mg2+
weak, above 4 mM, activates below
Bos taurus
additional information
no inhibition by EDTA, GSH, cysteamine or high phosphate concentrations
Bos taurus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.73
-
ATP
pH 8.5, competitive with AMP
Bos taurus
0.74
-
ATP
pH 8.5, competitive with GTP
Bos taurus
0.77
-
GDP
pH 8.5
Bos taurus
0.8
-
GTP
pH 8.5
Bos taurus
0.9
-
ADP
pH 8.5
Bos taurus
1.6
-
AMP
pH 8.5
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
GDP
pH 8.0, with ADP
Bos taurus
0.033
-
AMP
-
Bos taurus
0.056
-
GTP
pH 8.0, with AMP
Bos taurus
0.29
-
ADP
pH 8.0, with GDP
Bos taurus
0.63
-
ITP
pH 8.0, with AMP
Bos taurus
1
-
ATP
pH 8.0, with of AMP
Bos taurus
7.4
-
UTP
pH 8.0, with of AMP
Bos taurus
9.1
-
CTP
pH 8.0, with of AMP
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
matrix
Bos taurus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, 50% as effective as Mg2+
Bos taurus
Mg2+
activation, 4 mM, as good as Mn2+, slightly inibitory above 4 mM
Bos taurus
Mn2+
activation, as good as Mg2+
Bos taurus
additional information
no activation by EDTA, cysteamine, GSH or high phosphate concentrations
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
135
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, partially purified preparation, several weeks
Bos taurus
room temperature, 1 week
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + AMP
poor substrate, highly specific for AMP, phosphorylation at about 5% the rate of ITP
642534
Bos taurus
ADP
-
-
-
r
CTP + AMP
highly specific for AMP, phosphorylation at about 11% the rate of ITP
642534
Bos taurus
CDP + ADP
-
-
-
r
GTP + AMP
highly specific for AMP and ADP, in reverse reaction
642534
Bos taurus
GDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
GTP + AMP
phosphorylation at about 90% the rate of ITP
642534
Bos taurus
GDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
best substrate
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
highly specific for AMP and ADP in reverse reaction
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
no acceptor substrates are 3'-GMP
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
ITP + AMP
not: IMP, CMP, UMP
642534
Bos taurus
IDP + ADP
via nucleotide-enzyme complex, no phosphorylated enzyme intermediate
642534
Bos taurus
r
UTP + AMP
highly specific for AMP, phosphorylation at about 19% the rate of ITP
642534
Bos taurus
UDP + ADP
-
-
-
r
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
35% ammonium sulfate, AMP or other substrates enhance thermal stability
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.7
about half-maximal activity at pH 7 and 9.7
Bos taurus
Other publictions for EC 2.7.4.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737551
Kong
Differential expression of ade ...
Homo sapiens
Arch. Biochem. Biophys.
533
11-17
2013
-
-
-
-
1
-
-
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1
-
-
1
-
1
-
-
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-
-
2
-
-
1
-
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-
-
-
-
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-
-
-
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-
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-
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-
-
1
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1
-
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1
-
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-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
721237
Law
Expression, purification, crys ...
Plasmodium falciparum
Acta Crystallogr. Sect. F
68
671-674
2012
-
-
1
1
-
-
-
-
1
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722281
Ma
Subcellular localization of ad ...
Plasmodium falciparum
FEBS Lett.
586
3037-3043
2012
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
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-
1
-
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-
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-
-
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1
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662361
Gu
A GTP:AMP phosphotransferase, ...
Saccharomyces cerevisiae
J. Biol. Chem.
280
18604-18609
2005
-
-
1
-
-
4
-
-
1
-
-
-
-
2
-
-
1
-
1
-
1
-
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
663014
Yang
Molecular cloning and characte ...
Clonorchis sinensis
Parasitol. Res.
95
406-412
2005
-
-
1
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
662798
Ulschmid
Adenylate kinase and GTP:AMP p ...
Plasmodium falciparum
Mol. Biochem. Parasitol.
136
211-220
2004
-
-
1
-
-
-
4
3
-
1
1
-
-
4
-
-
1
-
-
-
1
-
7
-
-
-
-
-
1
-
-
-
3
-
-
-
-
1
-
-
-
-
-
4
3
3
-
1
1
-
-
-
-
1
-
-
1
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
642539
Xu
Characterization of human aden ...
Homo sapiens
Genomics
13
537-542
1992
-
-
1
-
-
-
-
-
-
-
-
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1
-
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-
-
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1
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-
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-
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-
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-
-
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-
-
-
-
-
-
-
642537
Diederichs
Three-dimensional structure of ...
Bos taurus
Biochemistry
29
8138-8144
1990
-
-
-
1
-
-
1
-
1
1
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
1
-
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1
-
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1
1
1
-
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-
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1
-
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
642536
Wieland
The amino acid sequence of GTP ...
Bos taurus
Eur. J. Biochem.
143
331-339
1984
-
-
-
-
-
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-
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1
1
1
1
-
2
-
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1
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2
-
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-
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1
1
1
1
-
-
-
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642535
Pai
Preliminary X-ray studies on t ...
Bos taurus
J. Mol. Biol.
164
347-350
1983
-
-
-
1
-
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-
-
1
1
1
1
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1
-
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1
-
-
2
-
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1
-
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1
1
1
1
-
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1
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2
-
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-
-
-
-
-
-
-
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-
-
642534
Albrecht
Purification and properties of ...
Bos taurus
Biochemistry
9
2462-2470
1970
-
-
-
-
-
3
7
8
2
4
1
-
-
2
-
-
1
1
-
2
1
2
9
-
-
-
1
-
1
1
-
-
6
-
-
-
-
-
-
-
-
3
-
7
6
8
2
4
1
-
-
-
-
1
-
2
1
2
9
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
642538
Chiga
Nucleotide transphosphorylases ...
Sus scrofa
J. Biol. Chem.
236
1800-1805
1961
-
-
-
-
-
-
4
-
-
2
-
-
-
2
-
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1
-
-
2
1
1
6
-
1
-
3
-
1
1
-
-
-
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-
-
-
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
1
-
2
1
1
6
-
1
-
3
-
1
1
-
-
-
-
-
-
-
-
642540
Heppel
Nucleoside monophosphate kinas ...
Bos taurus
Biochim. Biophys. Acta
32
422-430
1959
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-
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-
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1
-
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1
-
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1
1
1
7
-
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1
1
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-
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-
-
-
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-
-
-
-
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-
1
-
-
-
-
-
-
-
1
1
1
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-